Veterynarna biotehnologija – Veterinary biotechnology, 2020, 36, 146-154 [in Ukrainian]. https://doi.org/10.31073/vet_biotech36-15
Research Station of Epizootology of the IVM NAAS
BIOCHEMICAL PARAMETERS OF COWS BLOOD IN DIFFERENT PHYSIOLOGICAL PERIODS AND THEIR RELATIONSHIP WITH THE OBSTETRICS PATHOLOGY
Introduction. The study of biochemical parameters of blood allows us to study reliably the course of metabolic processes in the body of animals, with high accuracy to assess not only the general state of the body, but also to predict complications, to correct their prevention and to develop a plan of preventive therapy. This is especially important in the early stages of pregnancy, during the dry period and especially during calving.
The goal of the work was to investigate the peculiarities of the biochemical status of cows in different physiological periods and to establish its relationship with the development of obstetric pathology.
Materials and methods. The presence of metabolic disorders in cows was determined during the dry period, calving and in 10 days after calving by biochemical parameters of serum samples, which were studied by conventional methods using biochemical analyzer Cobas c 311 and content of inorganic elements – by atomic adsorption spectrophotometer 115M1. In 2016-2018, 865 cow's serum samples from six farms were examined.
Results of research and discussion. It was found that in the group of dry cows lack of total calcium (14,7% p≤0,001), inorganic phosphorus (9,0% p≤0,05), zinc (7,8% p≤0,05), cooper (18,6% p≤0,01), selenium (50,9% p≤0,001), iodine (33,9% p≤0,01) and cobalt (26,8% p≤0,01), and ferum excess (3,5 times p≤0,01) compared to the lower reference level, indicated the development of postpartum paresis in cows. In addition, the deficiency of these elements in cows' organism during dry period leads to abortion and stillbirths. It was registered selenium (36.7% p≤0.01), iodine (39.7% p≤0,01) and vitamin E (20.0% p≤0.01) significant deficiency in cows' after calving compared to lower reference level that leads to the development of subinvolution of the uterus and was one of the causes of postpartum endometritis and mastitis. The lack of zinc (12.4 % p≤0,05), selenium (29.2 % p≤0,01) and cobalt (27.6 % p≤0.01) in cows in 10 days after calving that were one of the reasons of delayed heat and low fertility, followed by early embryonic mortality.
Conclusion and prospects for further research. The cows of the dry period had low levels of total protein, glucose, total calcium, inorganic phosphorus, zinc, cooper, manganese, selenium, iodine and cobalt, which led to the development of postpartum paresis, retention of placenta and ketosis. In cows after calving, the decrease of total cholesterol indicated the development of subinvolution of the uterus, and in 10 days after calving deficiency of zinc, cobalt, inorganic phosphorus, and selenium content decreased, which created the basis for heat delay, low fertility. In the future, it is planned to investigate feed used for cattle feeding, protein, fat, carbohydrate, micro- and macronutrient content, and to balance the rations according to the results of the research.
Keywords: dry period, calving, postpartum period, biochemistry, vitamins, micro-macronutrients.