Bulletin "Veterinary biotechnology"

Veterynarna biotehnologija – Veterinary biotechnology, 2020, 36, 146-154 [in Ukrainian]. https://doi.org/10.31073/vet_biotech36-15

SACHUK R.M., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.

Research Station of Epizootology of the IVM NAAS


Introduction. The study of biochemical parameters of blood allows us to study reliably the course of metabolic processes in the body of animals, with high accuracy to assess not only the general state of the body, but also to predict complications, to correct their prevention and to develop a plan of preventive therapy. This is especially important in the early stages of pregnancy, during the dry period and especially during calving.

The goal of the work was to investigate the peculiarities of the biochemical status of cows in different physiological periods and to establish its relationship with the development of obstetric pathology.

Materials and methods. The presence of metabolic disorders in cows was determined during the dry period, calving and in 10 days after calving by biochemical parameters of serum samples, which were studied by conventional methods using biochemical analyzer Cobas c 311 and content of inorganic elements – by atomic adsorption spectrophotometer 115M1. In 2016-2018, 865 cow's serum samples from six farms were examined.

Results of research and discussion. It was found that in the group of dry cows lack of total calcium (14,7% p≤0,001), inorganic phosphorus (9,0% p≤0,05), zinc (7,8% p≤0,05), cooper (18,6% p≤0,01), selenium (50,9% p≤0,001), iodine (33,9% p≤0,01) and cobalt (26,8% p≤0,01), and ferum excess (3,5 times p≤0,01) compared to the lower reference level, indicated the development of postpartum paresis in cows. In addition, the deficiency of these elements in cows' organism during dry period leads to abortion and stillbirths. It was registered selenium (36.7% p≤0.01), iodine (39.7% p≤0,01) and vitamin E (20.0% p≤0.01) significant deficiency in cows' after calving compared to lower reference level that leads to the development of subinvolution of the uterus and was one of the causes of postpartum endometritis and mastitis. The lack of zinc (12.4 % p≤0,05), selenium (29.2 % p≤0,01) and cobalt (27.6 % p≤0.01) in cows in 10 days after calving that were one of the reasons of delayed heat and low fertility, followed by early embryonic mortality.

Conclusion and prospects for further research. The cows of the dry period had low levels of total protein, glucose, total calcium, inorganic phosphorus, zinc, cooper, manganese, selenium, iodine and cobalt, which led to the development of postpartum paresis, retention of placenta and ketosis. In cows after calving, the decrease of total cholesterol indicated the development of subinvolution of the uterus, and in 10 days after calving deficiency of zinc, cobalt, inorganic phosphorus, and selenium content decreased, which created the basis for heat delay, low fertility. In the future, it is planned to investigate feed used for cattle feeding, protein, fat, carbohydrate, micro- and macronutrient content, and to balance the rations according to the results of the research.

Keywords: dry period, calving, postpartum period, biochemistry, vitamins, micro-macronutrients.


  1. Dolecki, S. (2010). Wpáyw skáadu mineralnego pssz na gospodarkc u krów mlecznych w róinych regionach Ukrainy. Pasze Przemysáowe, 54-57 [in Polish].
  2. Sachuk, R.M., Katsaraba, O.A., Dmytriv, O.Ya. & Stravskyi, Ya.S. (2018). Diahnostyka metabolichnykh zrushen v orhanizmi koriv u period sukhostoiu ta rozrobka preventyvnykh zakhodiv [Diagnosis of metabolic changes in the body of cows during the dry period and the development of preventive measures]. Naukovi horyzonty – Scientific horizons, 9-10 (71), 69-74 [in Ukrainian].
  3. Sachuk, R.M., Zhyhaliuk, S.V., Stravskyi, Ya.S., Katsaraba, O.A., Mahrelo, N.V., & Nikitinskyi, P.A. (2019). Diahnostyka metabolichnykh porushen v orhanizmi koriv pid chas otelennia ta rozrobka preventyvnykh zakhodiv [Diagnosis of metabolic disorders in the body of cows during calving and the development of preventive measures]. Naukovi horyzonty – Scientific horizons, 6 (79), 59-64 [in Ukrainian].
  4. Kravchenko, N.O. (2015). Diahnostyka metabolichnykh zrushen v orhanizmi velykoi rohatoi khudoby molochnoho napriamu produktyvnosti za umov sylosno-kontsentratnoho typu hodivli [Diagnostics of metabolic changes in organism of cattle of dairy cattle productivity in the conditions of silage-concentrate type of feeding]. Naukovo-tekhnichnyi biuleten Derzhavnoho naukovo-doslidnoho kontrolnoho instytutu veterynarnykh preparativ ta kormovykh dobavok i Instytutu biolohii tvaryn – Scientific and technical bulletin of State scientific research control institute of veterinary medical products and fodder additives and institute of animal biology, 16 (2), 157-162 [in Ukrainian].
  5. Stravskyi, Y.S. (2017). Diahnostyka, likuvannia ta profilaktyka akusherskoi patolohii u koriv (metodychni rekomendatsii) [Diagnosis, treatment and prevention of obstetric pathology in cows (guidelines)]. Lviv [in Ukrainian].
  6. Alekhin, Yu.N. (2011). Bolezni pecheni u vysokoproduktivnykh korov (diagnostika. profilaktika i terapiya) [Liver diseases in highly productive cows (diagnostics, prevention and therapy)]. Veterinariya – Veterinary medicine, 6, 3-7 [in Russian].
  7. Kovalenko, L.V., Rudenko, O.P., Boiko, V.S., & Krotovska, Yu.M. (2015). Diahnostyka metabolichnykh porushen u velykoi rohatoi khudoby. [Diagnosis of metabolic disorders in cattle]. Veterynarna medytsyna – Veterinary medicine, 101, 166-168 [in Ukrainian].
  8. Doletskyi, S.P. (2015). Teoretychne ta kliniko-eksperymentalne obgruntuvannia profilaktyky porushen mineralnoho obminu v koriv u bioheokhimichnykh zonakh Ukrainy [Theoretical and clinical and experimental substantiation of the prevention of disorders of mineral exchange in cows in biogeochemical zones of Ukraine]. Extended abstract of Doctor's thesis, Kyiv [in Ukrainian].
  9. Levchenko, V.I., Vlizlo, V.V., & Kondrakhin, I.P. (2004). Klinichna diahnostyka vnutrishnikh khvorob tvaryn [Clinical diagnostics of internal animal diseases]. Bila Tserkva [in Ukrainian].
  10. Pokrovskiy, A.A. (Ed.). (1969). Biokhimicheskiye metody issledovaniy v klinike [Biochemical research methods in the clinic]. Mosсow: Medytsyna [in Russian].
  11. Antonova, B.Y. (Ed.). (1989). Laboratornyye issledovaniya v veterinarii [Laboratory studies in veterinary medicine]. Moscow: Agropromizdat [in Russian].
  12. Rokitsky, P.F. (1973). Biologicheskaya statistika [Biological statistics]. Minsk: Higher School [in Russian].

Download full text in PDF