Bulletin "Veterinary biotechnology"

Veterynarna biotehnologija – Veterinary biotechnology, 2021, 39, 9-17 [in Ukrainian]. https://doi.org/10.31073/vet_biotech39-01

AYSHPUR O.Y., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., MUSHTUK I.Yu., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., SHEREMET N.O., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., BEREZOVCHUK S.M., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., GUMENYUK V.V., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., ERMOLENKO O.M., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., DEREVIANKO M.M., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.

Institute of Veterinary Medicine NAAS

STUDY OF ANTIBIOTIC RESISTANCE OF ENTEROCOCCUS

Introduction. Enterococci are facultative anaerobic asporogenic chemoorganotrophic gram-positive bacteria. According to the previous classification (until 1984) belonged to class D streptococci. According to the current situation, the genus Enterococcus. These microorganisms are sorbents of the intestines of humans, animals and birds. The main symbionts of the intestinal microflora, for example, humans, are two types of enterococci: E. faecalis (90-95%) and E. faecium (5-10%), and their pathogenic strains can cause a whole list of infections, which increases every year. A number of scientists report the isolation of vancomycin-resistant enetrococci (BRE) in their intestines of cattle, pigs, sheep, and chickens. Thus, the study of antibiotic resistance of enterococci is relevant, including their importance as a reservoir of resistance genes.

The goal of our work was to study the antibiotic resistance of enterococci, including vancomycin-resistant strains of enterococci isolated from biomaterials from animals.

Materials and methods. The work was carried out on the basis of the laboratory of bacterial diseases of animals of the Institute of Veterinary Medicine of NAAS, on livestock farms and poultry farms during 2018-2021. Determination of susceptibility to antimicrobial drugs was performed on Mueller-Hinton medium (HiMedia, India) by disco-diffusion method according to the Bauer-Kirbi method using standard commercial disks with antibacterial drugs. Preparation of microbial suspensions was performed according to the optical turbidity standard of 1.0 units on the McFarland scale.

Results of research and discussion. In our research 1017 samples of biomaterials (milk, vaginal washings, samples of feces, washings of nasal mucus) from cows of patients with mastitis, endometritis were investigated; from calves with diarrhea and respiratory syndrome, pathological material from forcibly slaughtered and dead pigs and chickens. 30 species of microorganisms were identified, among which the percentage of enterococci ranged from 16.3% to 18.2%. The most number of Enterococcus spp isolates showed resistance to vancomycin (59.3%) and kanamycin (59.2%). The isolates were also resistant to amoxicillin (55.9%), rifampicin (54.2%), tetracycline (53.7%) and erythromycin (50.0%). The lowest number of isolates was sensitive to cefotaxime (17.3%). The largest number of isolated isolates of E. fecalis were not sensitive to tetracycline (62.5%), kanamycin (56.0%) and erythromycin (46.9%). The smallest number of isolates of this species of bacteria showed resistance to danofloxacin (24.5%). For other antibiotics used, the number of E. fecalis isolates that showed resistance ranged from 30.0% to 37.5%. Two isolates of E. faecium from three showed resistance to amoxicillin, erythromycin, chloramphenicol and tetracycline, which amounted to 66.7%.

Conclusions and prospects for further research. From the studied 1017 samples of were identified up to 30 species of microorganisms, among which the percentage of enterococci ranged from 16.3 to 18.2%.The largest number of Enterococcus spp isolates showed resistance to vancomycin (59.3%) and kanamycin (59.2%). The largest number of E. fecalis isolates were not sensitive to tetracycline (62.5%), kanamycin (56.0%) and erythromycin (46.9%), and isolates of E. faecium showed resistance to amoxicillin, erythromycin, chloramphenicol and tetracycline (66.7% each). In the future, it is important to continue monitoring the antibiotic resistance of clinical strains of enterococci and to study the genetic features of the acquired resistance of these bacteria to antibacterial drugs, including the presence of plasmids and to determine their role.

Keywords: bacterias, enterococci, antibiotic resistance, vancomycin-resistant strains.

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