Bulletin "Veterinary biotechnology"

Veterynarna biotehnologija – Veterinary biotechnology, 2021, 39, 44-52 [in Ukrainian]. https://doi.org/10.31073/vet_biotech39-04

ZAKHAROVA O., е-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., TARASOV O.,канд. вет. наук, е-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., YANGOL Y.,е-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.

Institute of Veterinary Medicine NAAS

USE OF POLIMERASE CHAIN REACTION FOR DETECTION OF TOXINPRODUCING FUNGI OF FUSARIUM GENUS

Introduction. The mycotoxicosis issues due to the  wide area of disseminationof toxinogenic fungi is relevant because of the spread of poisoning animals with fusariotoxins. According to the published data, 80% of cereals are contaminated with fusarium toxins. As a result of long-term monitoring of micotoxins containmination of seed, food and grain, it was found that 60% of them were containminated, and 12% contained fusariotoxins.

The goal of the work was to detect of toxin-producing species of the genus Fusarium isolated in Ukraine by polymerase chain reaction (PCR).

Materials and methods. It was used 24 isolates of Fusarium genus fungi from museum of the Institute of veterinary medicine NAAS. Studies of morphological and cultural properties were performed using conventional microbiological methods.

The fusariotoxin produsing isolates were determined by multiplex PCR test (conventional method with electrophoretic reading of results) using three sets of primers recommended by Bluhm et al. (2004). The assay parameters were optimized by us.

The results of experimental studies are processed by conventional methods of statistics.

Results of research and discussion. According to the analysis of the results of microbiological studies, it was found that all the studied isolates had the main typical properties characteristic of microscopic fungi of the genus Fusarium spp.

In our study, the multiplex PCR we detected among the 24 isolates isolated from corn grain obtained from Kyiv, Cherkasy, Zhytomyr and Chernihiv regions, 5 isolates were carrying the gene associated with the production of fumonisin (20.8%) and 2 (8.3%) isolates were associated with trichothecene production.

Conclusions and prospects for further research:

1. As a result of research, it was found that the use of multiplex PCR allows to determine the presence of toxin-producing microscopic fungi of the genus Fusarium.

2. Analysis of research results showed that among 24 isolates isolated from corn grain obtained from Kyiv, Cherkasy and Kherson regions, 5 isolates produced fumonisin (20.8%) and 2 (8.3%) isolates – trichothecene.

The use of PCR to detect toxin-producing microscopic fungi of the genus Fusarium in Ukraine is important for improving measures to prevent poisoning of animals with mycotoxins and improve the quality of livestock products.

Keywords: Fusarium, fumonisine, PCR, isolate, toxigenic strain.

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