Bulletin "Veterinary biotechnology"

Veterynarna biotehnologija – Veterinary biotechnology, 2021, 39, 53-64 [in Ukrainian]. https://doi.org/10.31073/vet_biotech39-05

MAZANNYІ O.1, 2, e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., PRYKHODKO YU.1, 2, e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., NIKIFOROVA O.1, e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., MAZANNA M.1, e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., FEDOROVA H.1, e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.

1 State Biotechnology University
Institute of Veterinary Medicine NAAS

EFFECT OF «NEMASECTIN», «BROVERMECTIN™ GEL» AND «HELMISAN®» ON EQUINE BLOOD COUNTS

Introduction. Measures to control helminthoses include the use of animal-specific chemotherapeutic preparations aimed at destroying parasites. The effectiveness of the means mainly depends on the activity of the active substance, etc.

The goal of the work was to determine experimentally the safety of anthelmintic treatments and the effect of various active anthelmintic substances on the body of horses.

Materials and methods. The study was conducted in 2020 on animals of the equestrian club «Impulse» (Poltava region). The effect of «Nemasectin», «Brovermectin™ gel» and «Helmisan®» on the body of horses was studied. The number of erythrocytes, leukocytes, hemoglobin content was determined, the leukocyte formula was calculated, the activity of alanine aminotransferase (ALT), aspartate aminotransferase (AST), the amount of urea and creatinine were measured.

Results of research and discussion. To study the effect of anthelmintics with different composition of active substances on the body of horses, clinically healthy animals were treated with preparations and laboratory blood tests were conducted. In the blood of horses treated with «Nemasectin», «Brovermectin™ gel» and «Helmisan®» the hemoglobin content has increased in a one day after administration of preparations, but on the 4th day after deworming hemoglobin decreased to 146.50±3.66 g/l, 145.67±2.60 g/l (p≤0.05), 229.23±1.18 g/l (p≤0.05), respectively. Analysis of leukocyte count in all animals showed neutropenia, monocytosis and lymphocytosis before applying preparations. Indicators of the number of monocytes and lymphocytes remained high in a one day after the administration of anthelmintics, and has returned to the normative indicators and indicators of animals from the control group on the 4th day.

In the blood of animals treated with «Nemasectin», the activity of ALT and AST during the experiment increased within normal limits, and the amount of urea and creatinine, on the contrary, decreased during the experiment. In the blood of animals treated with «Brovermectin™ gel» revealed a steady increase in the activity of only AST to 553.67±3.18 units/l (p≤0.01) compared with indicators before deworming. And in animals treated with «Helmisan®» revealed an increase in ALT activity to 35.85±1.79 units/l (p≤0.01).

Conclusions and prospects for further research. On the 4th day of the experiment, the results of a clinical blood study of horses of the experimental groups, which were given «Nemasectin», «Brovermectin™ gel» and «Helmisan®», were within the normative parameters and did not exceed the parameters of the control group. In horses treated with «Nemasectin», biochemical parameters were within normal limits during the experiment. «Brovermectin™ gel» caused an increase in AST activity in the serum of horses by 1.4 times (p≤0.01), and «Helmisan®» – an increase in ALT activity by 1.5 times (p≤0.01), compared with indicators before deworming. In the future, it is necessary to continue the study of the effects of antiparasitic preparations on the body of horses, in order to adequately assess the preparations used during the planned deworming.

Keywords: «Nemasectin», «Brovermectin™ gel», «Helmisan», horses, blood, morphological and biochemical studies.

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