Bulletin "Veterinary biotechnology"

Veterynarna biotehnologija – Veterinary biotechnology, 2021, 39, 108-116 [in Ukrainian]. https://doi.org/10.31073/vet_biotech39-10

SYTIUK M., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.

Institute of Veterinary Medicine NAAS

PHYLOGENETIC ANALYSIS OF THE COMPLETE GENOME OF SECOND-TYPE CIRCOVIRUS DNA SAMPLES IDENTIFIED FROM WILD PIGS IN UKRAINE

Introduction. Circovirus infection of pigs is caused by circovirus of the second type, which in terms of epizootology is common on all continents of the world.

The goal of the work. Тo carry out sequencing of genetic samples of circovirus of pigs of the second type isolated from wild pigs and to compare them by phylogenetic analysis with the existing isolates of this virus.

Materials and methods. For sequencing of the complete genome of circovirus of the second type, 23 samples were selected with maximum coverage of the territory of Ukraine – 18 districts of 12 oblasts.  Sequencing of the genome of 23 isolates of porcine circovirus of the second type was performed at the National Veterinary Institute in Pulawy, Poland on two fragments of primers CBB1-CBB2 and CSZ2-CBB3. Phylogenetic analysis was performed based on DNAstar (Lasergene) and CLC DNA Workbench (CLC bio).

Results of research and discussion. According to the results of the research23 samples genetic material were suitable for sequencing of the whole genome of circovirus of the second type.

Phylogenetic analysis of 23 circovirus isolates of the second type showed that the strains isolated from Ukrainian wild pigs belong to both CVS 2 genotypes, where 16 sequences belong to group 1A/1B, two sequences to group 1C, four sequences to group 2D. Thus, the cluster distribution of sequenced samples from feral pigs showed their proximity and genetic affinity with circovirus isolates of the second type isolated in other countries. Thus, in addition to the determined seroprevalence of wild pigs of Ukraine to the circovirus of pigs of the second type, our scientific research also confirmed the presence of circulation of this virus in these representatives of wild fauna.

Conclusions and prospects for further research. Thus, the results of molecular genetic studies showed that isolates of circovirus of the second type isolated from wild pigs of Ukraine by phylogenetic analysis are closely related to isolates isolated from Canada, Japan, China, Vietnam, Taiwan, France, Hungary, Poland, the Netherlands, Serbia.

Given the above data, in the perspective of further scientific work it is necessary to focus on proving the fact of transmission of circovirus isolates of the second type from domestic pigs to feral pigs and vice versa with the involvement of phylogenetic analysis.

Keywords: circovirus type 2, wild boars, molecular genetic studies.

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