Bulletin "Veterinary biotechnology"

Veterynarna biotehnologija – Veterinary biotechnology, 2021, 39, 145-155 [in Ukrainian]. https://doi.org/10.31073/vet_biotech39-13

CHECHET O.M. , e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.

State Scientific and Research Institute of Laboratory Diagnostics and Veterinary and Sanitary Expertise


Introduction. Due to pathogenic microorganisms resistance and their ability to adapt to disinfectants, the issue of their rotation and timely change of disinfectants based on various active substances are relevant.

The goal of the study is to investigate and compare common disinfectants in poultry farming and select the most optimal active substances for the creation of biocides for aerosol application.

Materials and methods. In order to determine the effectiveness in the laboratory, the following disinfectants were selected: Vrcon C, VTS 885, Virocid, Biocontact as well as new experimental drugs: Diolide, Biolide. The average application concentrations were used, which are determined according to the manufacturers leaflets as 0.3% for the 60-minute exposure.

Test cultures of Escherichia coli UNCSM - 007 (Escherichia coli ATCC 25922) were used to assess bactericidal activity in experimental disinfectants. The effectiveness of the new disinfectants was determined by the presence or absence of growth of opportunistic pathogenic microflora on surfaces of the test objects.

Results of research and discussion. In order to choose a disinfectant, properly special attention is paid to the cost of surface treatment with the same disinfection efficiency. Septodor forte, Virocid and others have a prolonged effect and the cost of their use, depending on the treatment method, is high. In addition, they also contain glutaraldehyde, which is toxic. Experimental disinfectants Biolide and Diolide have a universal and economic effect, which allows to use them in the various treatment schemes in the presence of animals.

According to the results of research, we found that the created substances Diolide, in 0.1 & 0.5% concentration, and Biolide, in 0.3 & 1.0% concentration, at an exposure of 30 – 60 min completely neutralize E. coli, while other drugs neutralized the test cultures at higher concentrations and at longer exposures.

Conclusions and prospects for further research:

1. It was established that the efficiency indicators for the using of the newest disinfectants in the laboratory are not inferior to those of commonly used. Thus, Diolide and Biolide in the recommended concentrations at an exposure of 30 minutes delay the growth of test cultures on smooth contaminated surfaces.

2. The selection of optimal concentrations for new disinfectants was carried out. It was found that the Diolide (concentration 0.1 & 0.5%) and Biolide (concentration 0.3 & 1.0%) at an exposure of 30 min completely neutralize E. coli.

The next stage of the research comprises the study of Diolide and Biolide disinfectants in a production environment on poultry farms in Ukraine.

Key words: Disinfectants, disinfection, test cultures, test objects, efficiency.


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