Bulletin "Veterinary biotechnology"

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Veterynarna biotehnologija – Veterinary biotechnology, 2022, 40, 32-42 [in Ukrainian]. https://doi.org/10.31073/vet_biotech40-03

GRISHCHUK G.P., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., GURALSKA S.V., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.

Polissya National University

HISTOMORPHOLOGY OF THE REPRODUCTIVE TRACT OF HEIFERS OF THE MATING AGE WITH SYMPTOMATIC INFERTILITY

Introduction. Infertility of cattle, in today’s conditions, unfortunately remains a very urgent problem. The paper presents the results of histological studies of the reproductive tract of heifers of mating age with symptomatic infertility.

Goal of the work. Establishment of histomorphological changes in the genitals of heifers of mating age in the symptomatic form of infertility.

Material and methods. The material for the study were the ovaries, fallopian tubes and uterus of 10 heifer.We used generally accepted methods of tissue fixation and sections reparation for histological studies.

Research results and discussion. The results of histological examination of the reproductive tract of heifers of the Ukrainian black-and-white breed of mating age with symptomatic infertility indicated that most of the cortex of the ovary did not contain follicles, butwas represented by fibrous connective tissue. We also observed the presence of individual large, empty follicles that did not reach maturity and underwent destructive restructuring, overgrowth of granulation tissue. Hypertrophy of folds and hyperplasia of mucosal epithelial cells were found in the fallopian tubes. In some areas of the uterus, the presence of ciliated cells was noted, and in the bends of the folds, the epithelium of the mucous membrane was absent, in the glandular layer of the mucous membrane – an overgrowth of connective tissue, in the vascular layer of the myometrium – mucoid swelling of the walls of blood vessels.

Conclusions and prospects for further research. According to the symptomatic infertility of heifers of mating age of the Ukrainian black-and-white breed, pathohistological changes in the ovaries, fallopian tube wall and uterus are noted. In the ovaries, a violation of the process of folliculogenesis is detected, due to pronounced structural changes in the cortical substance, which was expressed in the predominance of atresia over the process of follicle maturation. Epithelial proliferation was noted in the mucous membrane of the cervical isthmus. Detritus of destroyed cells and destruction of individual folds were observed in the lumen of the cervix. In the uterine wall, in the absence of significant changes in the glandular layer of the endometrium, there is an overgrowth of connective tissue, which indicates the organization of the uterine wall. In the future, it is planned to conduct an histochemical study of organs for symptomatic infertility of heifers.

Keywords: histological changes, ovary, fallopian tube, uterus, heifers.

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