Veterynarna biotehnologija – Veterinary biotechnology, 2022, 40, 61-69 [in Ukrainian]. https://doi.org/10.31073/vet_biotech40-06
Institute of Veterinary Medicine NAAS
EFFECT OF IMMUNOMODULATOR RIBOTAN ON THE INDICATORS OF CELLULAR IMMUNITY IN FOALS
Introduction.It is necessary to maintain a body at sufficient physiological level for the effective functioning of the immune system, which determines the resistance and immune reactivity of animals. The development of equine immunity from the fetus to adulthood is complex. The foal’s immune response and the available immune mechanisms along with changes over the first months of life until the immune system becomes adult-like are only partially understood. While several innate immune responses seem to be fully functional from birth, the onset of adaptive immune response is delayed.
The goal of the work was to study the cellular immunity of foals under the influence of immunomodulatorRibotan.
Materials and methods. 14-day-old foals (n = 20) were used for the study. Animals in the experimental group were administered the immunomodulator Ribotan intramuscularly in the middle third of the neck once a day at a dose of 1 cm3/animal for three days. Blood sampling was performed in 1-month old foals.
Resultsofresearchanddiscussion. Immunomodulators introduction for animals normalizes T-immune system, in particular, increases the number of leucocytes in the blood, lymphocytes ofcertain populations, especially theophyllinum-resistant subpopulation of T-helper cells, increases the functional activity of lymphocytes.
Results of the content of T-helper and T-suppressor cells in foal’s blood after Ribotan administration showed that the use of immunomodulators not only increases the number of T-helper cells, but restores the ratio T-h / T-s, which returned to the optimal rate (1.9).
In relation to thermostable T-lymphocytes, it was noted the trend to the most optimal level of these cells registered in foals after administration of ribotan (values within 3-4%). The increasing in number of thermostable T-cells more than 4% indicated an increase of suppressor T-cells population, indicating the inhibition of T-helper cells, and therefore the production of antibodies.
Conclusionsandprospectsforfurtherresearch. Thus, the use of ribotan in dose of
1 cm3/animal for three days leads to an increasing in 1.4-4.5% of the number of leukocytes in the blood of experimental group of foals compared with control animals. Under influence of ribotan in the blood of foals increases cell (number of T-lymphocytes in 0.4-2.3%) and functional activity (T-active lymphocytes in 2.3 times; P <0.05) of T-immune system.
Keywords: immunity, immunomodulator, ribotan, T-cells, foals.