Bulletin "Veterinary biotechnology"

  • Print

Veterynarna biotehnologija – Veterinary biotechnology, 2022, 40, 82-97 [in Ukrainian]. https://doi.org/10.31073/vet_biotech40-08

MEZHENSKA N.A.1, e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., MEZHENSKYI A.O.1, e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., NYCHYK S.A.1, e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., MEZHENSKYI A.A.2, e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., TKACHENKO S.M.2, e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.

1Institute of Veterinary Medicine NAAS

2National University of Life and Environmental Sciences of Ukraine

DEVELOPMENT OF A COMPLEX ANTISEPTIC PREPARATION BASED ON POLYHEXAMETHYLENEGUANIDINE HYDROCHLORIDE AND ISOPROPANOL FOR USE IN VETERINARY SURGERY

Introduction. The search for new means of preventing surgical infection is relevant for veterinary medicine. It is promising to study the possibility of using polyhexamethyleneguanidine hydrochloride (PHMG-HC) for the development of antiseptic preparations for preparing the surgical field and the surgeon’s hands.

The goal of the work.To study the toxic effect of aqueous solutions of PHMG-HC on the infusoria Tetrahymena pyriformis, to develop a complex antiseptic preparation based on PHMG-HC and isopropanol, and to investigate its local irritant effect and bactericidal activity.

Materials and methods. Determination of the possibility of using PHMG-HC for the development of an antiseptic preparation was determined by the degree of its toxic effect on the infusoria Tetrahymena pyriformis.

The complex preparation «ASEPT-VH» was made by mixing its components according to the recipe in the following sequence: isopropanol + distilled water + glycerin + PHMG-HC.

The bactericidal activity of the «ASEPT-VH» preparation was determined by the microbiological method on sterile calico test objects contaminated with test cultures of E. coli (strain 1257), S. aureus (strain 209-P) and Candida albicans.

The local irritant effect of the «ASEPT-VH» preparation on the skin of laboratory animals was determined in rabbits.

Research results and discussions. It has been established that 0.02–0.1% solutions of PHMG-HC at an exposure of 1–10 min do not have a toxic effect on the infusoria Tetrahymena pyriformis.

The antiseptic effect of the preparation «ASEPT-VH» is pronounced and depends on the exposure of the effect on the calico test objects contaminated with the test cultures of E. coli, S. aureus and Candida albicans.

It has been established that the «ASEPT-VH» preparation does not have a local irritating effect on the skin of rabbits and can be used to treat the surgical field of animals or to prepare the surgeon’s hands for surgery.

Conclusions and prospects for further research. An aqueous solution of PHMG-HC at a concentration of 0.02-0.1% belongs to the class of low-toxic substances. The developed antiseptic preparation «ASEPT-VH» does not have a local irritating effect on the skin of laboratory animals and, after a 5-minute exposure, completely disinfects calico test objects contaminated with test cultures of E. coli (strain 1257), S. aureus (strain 209-P) and Candida albicans, which indicates its high antimicrobial efficiency.

Prospects for further research are to conduct clinical trials of the developed preparation «ASEPT-VH» in the preparation of the surgical field for surgical operations in animals.

Keywords: antiseptic, polyhexamethyleneguanidine hydrochloride, isopropanol, «ASEPT-VH», veterinary surgery.

REFERENCES

  1. Vlasenko, V.M., Rublenko, M.V., Kozij, V.I., et al. (2005). Antyseptyka ta aseptyka u veterynarnij hirurgii [Antisepticsandasepsisinveterinarysurgery]. Bila Tserkva [in Ukrainian].
  2. Kovalenko, V.L., & Nedosjekov, V.V. (2011). Koncepcija rozrobky ta vykorystannja kompleksnyh dezinfektantiv dlja veterynarnoi medycyny: monografija [The concept of development and use of complex disinfectants for veterinary medicine: monograph]. Kyiv: NUBiP Ukrainy [in Ukrainian].
  3. Handbuch der Antiseptic (11th ed.). (1987). Berlin.
  4. Mandygra, M.S., Stepanjak, I.V., Lysycja, A.V., et al. (2008). Vykorystannja poligeksametylenguanidynu dlja dezinfekcii [Use of polyhexamethylene guanidine for disinfection]. Agrarnyj visnyk Prychornomorja. Zbirnyk naukovyh prac – Agrarian Bulletin of the Black Sea Coast. Collection of scientific works], 42 (2), 69-73 [in Ukrainian].
  5. Barkova, N.P. (1992). Rezultaty issledovanij perspektivnyh solej PGMG s celju vnedrenija v narodnoe hozjajstvo [Results of studies of promising salts of PHMG for the purpose of implementation in the national economy]. Angarsk: Sib. otd. RAMN [in Russian].
  6. Voinceva, I.I. (2009). Poliguanidiny – dezinfekcionnye sredstva i polifunkcionalnye dobavki v kompozicionnye materialy[Polyguanidines - disinfectants and polyfunctional additives in composite materials]. Moskva: LKM-press [in Russian].
  7. Gembickij, P.A., & Voinceva, I.I. (1998). Polimernyj biocidnyj preparat poligeksametilen guanidin[Polymeric biocidal preparation polyhexamethylene guanidine]. Zaporozhe: Poligraf [in Russian].
  8. Efimov, K.M., Gembickij, P.A., & Snezhko, A.G. (2000). Poliguanidiny – klass malotoksichnyh dezsredstv prolongirovannogo dejstvija [Polyguanidines – a class of low-toxic disinfectants of prolonged action]. Dezinfekcionnoe delo–Disinfection business, 4, 25-31 [in Russian].
  9. Barkova, N.P. (1989). Toksikologicheskie issledovanija perspektivnyh solej PGMG [Toxicological studies of promising PHMG salts]. Gigiena i sanitarija– Hygiene and sanitation, 2, 14-15 [in Russian].
  10. Mak-Donell, G. (2002). Antiseptiki i dezinficirujushhie veshhestva: aktivnost, dejstvie i rezistentnost[Antiseptics and disinfectants: activity, action and resistance]. Moskva [in Russian].
  11. Rotter, M.L. (1993). Chirurgische Handdesinfection. Klinische Antiseptik. Berlin, 67-82.
  12. Palij, T.K. (Ed.). (1997). Antyseptyka u profilaktyci j likuvanni infekcij [Antisepticsinpreventionandtreatmentofinfections]. Kyiv: Zdorovja [in Ukrainian].
  13. Kovalenko, V.L., & Tereshhenko, S.M. (2010). Pat. na korysnu model № 51142 Ukraina. Sposib ocinky toksychnosti dezinfikujuchogo preparatu z vykorystannjam infuzorii tetrahimeny piriformis [Method of assessing toxicity of disinfectant using ciliate tetrahymena pyriformis]. sis.ukrpatent.org. Retrieved from https://sis.ukrpatent.org/uk/search/detail/258703.
  14. Cikunij, A.D. (2001). Opredelenie jendogennoj intoksikacii po toksikourii s ispol'zovaniem Tetrachimena pyriformis [Determination of endogenous intoxication by toxicouria using Tetrachimena pyriformis]. Klin. lab. diagn. – Clinical laboratory diagnostics, 6, 50-52 [in Ukrainian].
  15. Everhart, L.P. (1972). Methods with Tetrahymena. Methods in cell physiology, 5, 219-288.
  16. Jaroshenko, M.O. (2005). Opredelenie parametrov ostroj toksichnosti decisa forte dlja krys pri peroral'nom vvedenii [Determination of parameters of acute toxicity of decis forte for rats when administered orally]. Veterinarna medicina: Mіzhvіd. tematich. nauk. zb. –Veterinary medicine: International thematic scientific collection, 85, 1182-1187 [in Ukrainian].
  17. Hmelnyckyj, G.O., Malynin, O.O., Kutsan, O.T., & Duhnyckyj, V.B. (2012). Veterynarna toksykologija: pidruchnyk [Veterinary toxicology: textbook]. Kyiv: Agrarna osvita [in Ukrainian].
  18. Kosenko, M.V., Malyk, O.G., Kocjumbas, I.Ja., et al. (1997). Toksykologichnyj kontrol novyh zasobiv zahystu tvaryn (Metodychni rekomendacii) [Toxicological control of new means of animal protection (Guidelines)]. Kyiv [in Ukrainian].
  19. Kovalenko, V.L. (2012). Teoretychne i eksperymentalne obgruntuvannja rozrobky ta vykorystannja kompleksnyh dezinfektantiv dlja veterynarnoi medycyny [Theoretical and experimental substantiation of development and use of complex disinfectants for veterinary medicine National Academy of Agrarian Sciences of Ukraine]. Doctor’s thesis. Kyiv [in Ukrainian].
  20. Afinogenov, G.E., Krasnova, M.V., & Afinogenova, A.G. (2008). Sravnenie metodov ocenki jeffektivnosti dezinfektantov i antiseptikov [Comparison of methods for evaluating the effectiveness of disinfectants and antiseptics]. Dezinfekcionnoe delo –Disinfection work, 4, 40-44 [in Russian].
  21. Arhipchuk, V.V., & Goncharuk, V.V. (2007). Kompleksnaja ocenka toksichnosti, cito- i genotoksichnosti poligeksametilenguanidina s ispolzovaniem rastitelnyh i zhivotnyh test-organizmov i ih kletok [Comprehensive assessment of toxicity, cyto- and genotoxicity of polyhexamethylene guanidine using plant and animal test organisms and their cells]. Himija i tehnologija vody – Chemistry and technology of water, 4, 357-369 [in Russian].
  22. Kolodij, G.V. (2018). Toksykologichne obgruntuvannja zastosuvannja dezinfikujuchogo zasobu na osnovi solej poligeksametylenguanidynu [Toxicological substantiation of the use of disinfectant based on salts of polyhexamethylene guanidine]. Candidate’s thesis. Lviv [in Ukrainian].
  23. Mandygra, M.S., & Lysycja, A.V. (2010). Analiz toksychnyh vlastyvostej poligeksametylenguanidynu [Analysis of the toxic properties of polyhexamethylene guanidine]. Naukovyj visnyk veterynarnoi medycyny–Scientific Bulletin of Veterinary Medicine, 6 (79), 9-12 [in Ukrainian].
  24. Kocjumbas, I.Ja., & Malyk, O.G. (2006). Doklinichni doslidzhennja veterynarnyh likarskyh zasobiv [Preclinical studies of veterinary drugs]. Lviv: Triada pljus [in Ukrainian].
  25. Jakubchak, O.M., Kovalenko, V.L., Homenko, V.I., Denysjuk, G.M., Bondar, T.O., & Midyk. S.V. (2005). Rekomendacii shhodo sanitarno-mikrobiologichnogo doslidzhennja zmyviv z poverhon test-objektiv ta objektiv veterynarnogo nagljadu i kontrolju: Metodychni rekomendacii [Recommendations for sanitary-microbiological examination of washes from the surfaces of test objects and objects of veterinary supervision and control: Guidelines]. Kyiv: NAU [in Ukrainian].
  26. Vysockij, A.Je. (2007). Metody toksikologicheskoj ocenki novyh dezinficirujushhih himiopreparatov, primenjaemyh v veterinarii [Methods of toxicological evaluation of new disinfectant chemotherapy drugs used in veterinary medicine]. Naukovo-tehnichnyj bjuleten Instytutu biologii tvaryn i DNDKI vet. preparativ ta kormovyh dobavok – Scientific and technical bulletin of the Institute of Biology of Creatures and DNDK vet. preparations and feed additives, 8 (3, 40), 344-352 [in Ukrainian].
  27. Panko, M.F., Ishhenko, V.D., Drobnycka, V.O., & Rudych, D.S. (2012). Miscevo-podraznjujucha dija novogo dezinfikujuchogo zasobu na osnovi poverhnevo aktyvnyh rechovyn i guanidynvmisnoi spoluky [Local irritant effect of a new disinfectant based on surfactants and guanidine-containing compounds]. Biologija tvaryn – Animal biology, 14, 1-2, 424-427 [in Ukrainian].
  28. Zapadnjuk, I.P., Zapadnjuk, V.I., Zaharija, E.A., et al. (1983). Laboratornye zhivotnye. Razvedenie, soderzhanie, ispolzovanie v jeksperimente [Uchebnoe posobie. laboratory animals. Breeding, maintenance, use in the experiment.Tutorial]. Kyiv: Vishha shkola [in Ukrainian].
  29. Kashin, A.S., Medvedeva, L.V., & Jurova, V.A. (2001). Mikroflora pri zazhivlenii laparotomnyh ran [Microflora in the healing of laparotomic wounds]. Veterinarija –Veterinary, 10, 35-38 [in Russian].

Download full text in PDF