Bulletin "Veterinary biotechnology"

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Veterynarna biotekhnolohiia – Veterinary biotechnology, 2022, 41, 69-80 [in Ukrainian]. https://doi.org/10.31073/vet_biotech41-07

MEZHENSKYI A.O., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., MEZHENSKA N.A., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., MEZHENSKYI A.A., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., NYCHYK S.A., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.

Institute of Veterinary Medicine of the NAAS

TKACHENKO S.M., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.

National University of Life and Environmental Sciences of Ukraine

 

ANTISEPTIC EFFECTIVENESS OF THE DRUG «ASEPT-VH» IN PREPARATION OF THE SURGICAL FIELD IN DOGS IN SURGERY

 Introduction. To prepare the surgical field, an «ASEPT-VH» preparation based on polyhexamethylene guanidine hydrochloride and isopropanol was developed. Under laboratory conditions, it has been proven that «ASEPT-VH» belongs to the class of slightly toxic substances, does not have a local irritant effect and exhibits high antimicrobial efficacy against E. coli, St. aureus and Candida albicans.

Thegoalofthework. To study the antiseptic efficacy of «ASEPT-VH» in the preparation of the surgical field in dogs under clinical conditions.

Materials and methods. The effectiveness of «ASEPT-VH» was compared with 5% alcohol solution of iodine and 70% ethanol in the preparation of the surgical field before ovariohysterectomy in dogs. We studied their effect on the resident microflora of the skin, the period of time during which the antiseptic activity of the drugs is preserved, as well as the development of purulent-inflammatory postoperative complications. The obtained digital results were processed by variational statistics.

Resultsofresearchanddiscussion. Preparation of the surgical field with 5% alcohol solution of iodine ensures its 100% sterility from anaerobic and aerobic microorganisms only during the first 5 minutes after treatment. 10 minutes after treatment, the growth of anaerobic and aerobic microorganisms is recorded. Complications in the postoperative period were not registered.

Preparation of the surgical field with the preparation «ASEPT-VH» ensures its sterility from anaerobic microorganisms from 5 to 50 minutes of observation, and sterility from aerobic microorganisms from 5 to 40 minutes of observation. Complications in the postoperative period were not registered.

Preparation of the surgical field with 70% ethanol does not ensure its sterility. Already 5 minutes after treatment, the growth of anaerobic and aerobic microorganisms was recorded. In two dogs, suppuration of the surgical wound developed in the postoperative period, which increased the period for removing surgical sutures to 9.8 days.

Conclusions and prospects for further research. The preparation «ASEPT-VH» in the preparation of the surgical field in dogs is more effective than 5% alcohol solution of iodine and 70% ethanol solution and can be used in practical work in veterinary clinics. In the future, we plan to determine the cost-effectiveness of the use of "ASEPT-VH" in the preparation of the surgical field.

Keywords: antiseptic, asepsis, «ASEPT-VH», operating field, veterinary surgery.

REFERENCES

  1. Vlasenko, V.M., Rublenko, M.V., Kozij, V.I. [ta in.] (2005). Antyseptyka ta aseptyka u veterynarnij hirurgii [Antiseptics and asepsis in veterinary surgery]. Bila Cerkva [in Ukrainian].
  2. Johnston, S.A., & Tobias K.M. (2017). Veterinary Surgery: Small Animal. (2th ed.). Saunders.
  3. Kashin, A.S., Medvedeva, L.V., & Jurova, V.A. (2001). Mikroflora pri zazhivlenii laparotomnyh ran [Microflora in the healing of laparotomic wounds]. Veterinarija – Veterinary, 10, 35-38 [in Russian].
  4. Rotter, M.L. (1993). Chirurgische Handdesinfection. Klinische Antiseptik. Berlin.
  5. Afinogenov, G.E., Blinov, N.P. (1987). Antiseptikavhirurgii [Antiseptics in surgery]. Leningrad: Medicina [in Russian].
  6. Mak-Donell, G. (2002). Antiseptiki i dezinficirujushhie veshhestva: aktivnost, dejstvie i rezistentnost[Antiseptics and disinfectants: activity, action and resistance]. Moskva [in Russian].
  7. Palij, T.K. [et al.]. (1997). Antyseptyka u profilaktyci j likuvanni infekcij [Antiseptics in prevention and treatment of infections]. Kyiv: Zdorovja [in Ukrainian].
  8. Mezhenska, N.A., Mezhenskyj, A.O., Nychyk, S.A., Mezhenskyj, A.A., & Tkachenko S.M. (2022). Rozrobka kompleksnogo antyseptychnogo preparatu na osnovi poligeksametylenguanidyn gidrohlorydu ta izopropanolu dlja vykorystannja u veterynarnij hirurgii [Development of a complex antiseptic preparation based on polyhexamethyleneguanidine hydrochloride and isopropanol for use in veterinary surgery]. Veterynarna biotehnologija – Veterinary biotechnology, Vol. 40, 82-97 [in Ukrainian].
  9. Dugdale, A. (Eds.). (2020). Veterinary Anaesthesia: Principles to Practice. (2th ed.). Wiley-Blackwell.
  10. Johnson, R.A., Snyder, L.B.C., & Schroeder, C.A. (2022). Canine and feline anesthesia and co-existing disease. (2th ed.). Hoboken, NJ: Wiley-Blackwell.
  11. Mezhenskyj, A.O., Petrenko, O.F. & Melnychuk, S.D. (2007). Premedykacija ta ocinka ii efektyvnosti u veterynarnij anesteziologii [Premedication and evaluation of its effectiveness in veterinary anesthesiology]. Vet. medycyna Ukrainy– Ukrainian veterinary medicine, Vol. 7, 30-34 [in Ukrainian].
  12. Mezhenskij, A.A. (2009). Monitoring anesteziologicheskogo obespechenija zhivotnyh v veterinarnoj hirurgii [Monitoring of anesthetic management of animals in veterinary surgery]. Veterinarija– Veterinary medicine, Vol. 7, 43-47 [in Russian].
  13. Jakubchak, O.M. [et al.]. (2010). Veterynarna dezinfekcija (instrukcija ta metodychni rekomendacii) [Veterinary disinfection (instructions and methodical recommendations)]. Kyiv: «Kompanija Bioprom» [in Ukrainian].
  14. Krasylnykov, A.P. (1993). Spravochnykpoantyseptyke [Handbook of antiseptics]. Mynsk: Vysh. shk. [in Belarussian].
  15. Afinogenov, G.E., Krasnova, M.V., & Afinogenova, A.G. (2008). Sravnenie metodov ocenki jeffektivnosti dezinfektantov i antiseptikov [Comparison of methods for evaluating the effectiveness of disinfectants and antiseptics]. Dezinfekcionnoe delo –Disinfection work, 4, 40-44 [in Russian].

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