Bulletin "Veterinary biotechnology"

Veterynarna biotekhnolohiia– Veterinary biotechnology, 2023, 42, 9-14 [in Ukrainian]. https://doi.org/10.31073/vet_biotech42-01

 HUDZ N., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., NYCHYK S., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.HLADIY M.*, e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.OBOZNENKO N., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., MELNYCHENKO O., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.

Institute of Veterinary Medicine of the NAAS

*National Academy of Agrarian Sciences of Ukraine



Introduction. Zoonotic diseases are a serious threat to public health worldwide. Leptospirosis remains one of the most common naturally occurring zoonotic infections worldwide.Ukraine is endemic for this disease. Currently the issue of cross-protection between Leptospira serovars in vaccinated animals has not been fully studied, so it is recommended to use vaccines based on serotype-specific antigen. Thus, the identification of dominant serovars of pathogenic leptospires is a necessary for ensuring effective measures to control and prevent the spread of this disease in Ukraine.

The goal of the work was to determine the prevalence of Leptospira serovars in cattle in 2020–2022.

Materials and methods. 225 serum samples from cattle suspected on leptospirosis were obtained from farms in 9 regions of Ukraine. Samples were tested in the microscopic agglutination test (MAT) using eight reference strains of Leptospira and eight serovars.

The serum samples were mixed with an equal volume of each of the Leptospira cultures. The samples with 1:50 titres or higher than considered positive in unvaccinated animals. Test was conducted using 1:50, 1:100, 1:500 and 1:2500 titersdilution. The results have been evaluated using a dark field microscope (× 400).

Results of research and discussion. 44.8% of 225 samples from cattle were positive. Most often detected Leptospira serovars were polonica (65.3%), kabura (54.5%) and icterohaemorrhagiae (33.7%).

The prevalence of only one serovar was observed in only 22.8% of samples and the majority of positive samples showed a reaction to several serovars at the same time – 77.2%. Mixed reactions to two serovars observed most often (31.7%), to three serovars less frequently (18.8%), and to four or more – in 5.0–8.9% of samples.

Polonica and kabura serovars dominated both in samples with seroprevalence to only one pathogen serovar (34.8% and 21.7% respectively) and mixed reactions (21.1% and 18.2% respectively).

Our results confirmed previously published data on the dominance of polonica and kabura serovars among cattle in Ukraine.

Conclusions and prospects for further research. The results of our research showed the prevalence of polonica and kabura serovars, which are 21.1% and 18.2% of all tested samples. Determining the dominant serovars of Leptospira among cattle is important for choosing the strategy for the immunoprophylaxis of the disease due to the serovar specificity of the protective effect of vaccines, for effective control of this disease.

Keywords: сattle, Leptospira, serovars, infection, MAT.


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