Bulletin "Veterinary biotechnology"

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Veterynarna biotekhnolohiia– Veterinary biotechnology, 2023, 43, 24-34 [in Ukrainian]. https://doi.org/10.31073/vet_biotech43-03

YEVSTAFIEVA V.1,2, e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., MELNYCHUK V.1,2, e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., BUDNYK D.1, e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., PRYKHODKO YU.2, e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., KYRYCHKO B.1, e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.

Poltava State Agrarian University

Institute of Veterinary Medicine of the National Academy of Agrarian Sciences of Ukraine

 

EFFECTIVENESS OF FLOTATION METHODS FOR COPROSCOPIC DIAGNOSIS OF NEMATODOSES OF THE GASTROINTESTINAL TRACT OF CATTLE

Introduction. Scientists believe that coproovoscopy methods are the most reasonable and ergonomic in the laboratory lifelong diagnosis of gastrointestinal parasitosis.In these methods, the feces of infected animals are used, which are examined for the detection of various parasitic germs. Today, not only the detection and identification of parasites, but also their quantitative determination plays a significant role.Moreover, the researchers claim that the quantitative determination of various parasitic elements takes a leading role in monitoring the epidemiological situation of infection in various territories, establishing the effectiveness of antiparasitic drugs when they are used.

The goal of the work was to establish the diagnostic efficiency of modern flotation methods of coproovoscopy for nematodoses of the gastrointestinal tract of cattle.

Materials and methods. We studied the diagnostic efficiency of methods of laboratory lifetime coproovoscopic diagnosis of nematodes of the alimentary canal of cattle, namely, using as a flotation liquid: a combined mixture of a solution of sugar and calcium nitrate (ratio 1: 1), ammonium nitrate, a saturated solution of sugar, a solution of urea. Examination of feces in the amount of 15 samples was carried out with exposures of the fecal suspension for 15 and 20 minutes.

Results of research and discussion. The conducted studies have established that in the laboratory diagnosis of strongylidoses of the digestive organs, trichurosis and strongyloidosis in cattle, the most effective method was coproovoscopy using a combined mixture of sugar solution and calcium nitrate with exposure for 20 minutes.This method provided 18.84–56.93% (p<0.001) higher rates of invasion intensity than methods where solutions of ammonium nitrate, sugar, and urea were used as flotation liquid.

Conclusions and prospects for further research:

1. The most effective method of lifetime laboratory diagnosis of strongylidoses of the digestive organs, trichurosis and strongyloidosis in cattle turned out to be the method of coproovoscopy with the use of a combined mixture of sugar solution and calcium nitrate as a flotation liquid for 20 minutes of exposure.

2. The use of a combined flotation solution, regardless of exposure, was more effective in the diagnosis of strongylidoses of the digestive organs in cattle by
16.32–35.58%, trichurosis by 26.41–70.60%, strongyloidosis by 23.07–53.85% than using solutions of ammonium nitrate, sugar and urea.

The results obtained by us regarding the effectiveness of modern methods of coproovoscopy for nematodoses of the gastrointestinal tract in cattle will be used in subsequent tests to determine the effectiveness of treatment measures for these helminthiasis.

Keywords: trichurosis, strongylidoses, strongyloidosis, laboratory diagnosis, coproovoscopy, effectiveness.

REFERENCES

  1. Roepstorff, A. (1998). Natural Ascaris suum infections in swine diagnosed by coprological and serological (ELISA) methods. Parasitology Research, 84 (7), 537-543. https://doi.org/10.1007/s004360050444.
  2. Pereckienė, A., Kaziūnaitė, V., Vyšniauskas, A., Petkevičius, S., Malakauskas, A., Šarkūnas, M., & Taylor, M. A. (2007). A comparison of modifications of the McMaster method for the enumeration of Ascaris suum eggs in pig faecal samples. Veterinary Parasitology, 149 (1-2), 111-116. https://doi.org/10.1016/j.vetpar.2007.04.014.
  3. Ward, M.P., Lyndal-Murphy, M., & Baldock, F.C. (1997). Evaluation of a composite method for counting helminth eggs in cattle faeces. Veterinary Parasitology, 73 (1-2), 181-187. https://doi.org/10.1016/s0304-4017(97)00060-5.
  4. McKenna, P.B. (1981). The diagnostic value and interpretation of faecal egg counts in sheep. New Zealand Veterinary Journal, 29 (8), 129-132. https://doi.org/10.1080/00480169.1981.34821.
  5. Nicholls, J., & Obendorf, D.L. (1994). Application of a composite faecal egg count procedure in diagnostic parasitology. Veterinary Parasitology, 52 (3-4), 337-342. https://doi.org/10.1016/0304-4017(94)90125-2.
  6. Melnychuk, V.V., & Yuskiv, I.D. (2019). Porivnialna efektyvnist sposobiv koproovoskopichnoi diahnostyky nematodoziv travnoho kanalu ovets [Comparative effectiveness of coproovoscopic diagnostics methods of sheep digestive tract nematodoses].Bulletin of Poltava State Agrarian Academy, 2, 197-203. https://doi.org/10.31210/visnyk2019.02.26 [in Ukrainian].
  7. Yevstafieva, V.O., Shendryk, L.I., Shendryk, Kh.M., Shendryk, I.M., & Huhosian, Yu.A. (2017). Udoskonalennia metodu kilkisnoho pidrakhunku lychynok nematod. [Improving method of quantitative counting larvae of nematodes]. Naukovo-Tekhnichnyi Biuleten NDTS Biobezpeky ta Ekolohichnoho Kontroliu Resursiv APK – Science and Technology Bulletin of SRC for Biosafety and Environmental Control of AIC, 5 (1), 120-123 [in Ukrainian].
  8. Yevstafіeva, V.O., Natjagla, I.V., & Melnychuk, V.V. (2016). Porivnjalna efektyvnist zazhyttjevyh sposobiv koproovoskopichnoi diagnostyky kapiljariozu kurej [Comparative efficiency of life-time methods of coproscopic diagnostics of capillary of chickens]. Visnyk Sumskogo Nacionalnogo Agrarnogo Universytetu – Bulletin of Sumy National Agrarian University, 11 (39), 150-154 [in Ukrainian].
  9. Dakhno, I.S., & Dakhno, Yu.I. (2010). Ekolohichna helmintolohiia [Environmental helminthology]. Sumy: Kozatskyi val» [in Ukrainian].
  10. Jacobs, D.E., Arakawa, A., Courtney, C.H., Gemmell, M.A., McCall, J.W., Myers, G.H., & Vanparijs, O. (1994). World Association for the Advancement of Veterinary Parasitology (W.A.A.V.P.) guidelines for evaluating the efficacy of anthelmintics for dogs and cats. Veterinary Parasitology, 52 (3-4), 179-202. https://doi.org/10.1016/0304-4017(94)90110-4.
  11. Cringoli, G., Rinaldi, L., Veneziano, V., Capelli, G., & Scala, A. (2004). The influence of flotation solution, sample dilution and the choice of McMaster slide area (volume) on the reliability of the McMaster technique in estimating the faecal egg counts of gastrointestinal strongyles and Dicrocoelium dendriticum in sheep. Veterinary Parasitology, 123 (1-2), 121-131. https://doi.org/10.1016/j.vetpar.2004.05.021.
  12. Mes, T.H.M., Ploeger, H.W., Terlou, M., Kooyman, F.N.J., Van Der Ploeg, M.P.J., & Eysker, M. (2001). A novel method for the isolation of gastro-intestinal nematode eggs that allows automated analysis of digital images of egg preparations and high throughput screening. Parasitology, 123 (3), 309-314. https://doi.org/10.1017/s0031182001008496.
  13. Cringoli, G., Rinaldi, L., Maurelli, M.P., & Utzinger, J. (2010). FLOTAC: new multivalent techniques for qualitative and quantitative copromicroscopic diagnosis of parasites in animals and humans. Nature Protocols, 5 (3), 503-515. https://doi.org/10.1038/nprot.2009.235.
  14. Bulashev, A., Akibekov, O., Tokpan, S., Serikova, S., & Abulgazimova, G. (2016). Hematological and serological investigation of dogs during experimental echinococcosis. Cardiovascular &amp; Hematological Agents in Medicinal Chemistry, 14 (1), 59-67. https://doi.org/10.2174/1871525714666160509130539.
  15. Crompton, D.W.T., & World Health Organization (‎2006)‎. Preventive chemotherapy in human helminthiasis: coordinated use of anthelminthic drugs in control interventions: a manual for health professionals and programme managers. World Health Organization. Retrieved from https://apps.who.int/iris/handle/10665/43545.
  16. Trach, V.N. (1981). Prostejshij metod vyjavlenija i ucheta jaic gelmintov v fekalijah zhivotnyh [The easiest method of identifying and addressing the helminth eggs in the feces of animals]: vtorаjа Zakavkazskajа konferencii po parazitologii (28–30 nojabrja 1979 hoda) –Proceedings of the second Zakavkazskoj conference on parasitology. (pp. 229-231). Erevan [in Russian].
  17. Starodub, Y.S. (2019). Improving coproovoscopic diagnostics of trichostrongylosis of geese. Bulletin of Poltava State Agrarian Academy, 1, 222-226. https://doi.org/10.31210/visnyk2019.01.26.
  18. Kotelnikov, G.A. (1984). Gelmintologicheskie issledovaniya zhivotnyh i okruzhayushej sredy [Helminthological studiesof animals and environment]. Moskva: Kolos [in Russian].
  19. Akbaev, M.S., Vasilevich, F.I., Akbaev, R.M., & Vodyanov, A.A. (1998). Parazitologija i invazionnye bolezni zhivotnyh [Parasitology andparasitic animal diseases]. Moskow [in Russian].
  20. Halat, V.F., Melnychuk, V.V., Yevstafieva, V.O., & Pruhlo, V.O. (2015). Patent na korysnu model № 100202. Ukraina. Sposib koproovoskopichnoi diahnostyky trykhurozu svynei [Copro-ovoscopic method for porcine trichuriasis diagnostics]. https://sis.nipo.gov.ua/uk/search/detail/879841/ [in Ukrainian].

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