Bulletin "Veterinary biotechnology"

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Veterynarna biotekhnolohiia – Veterinary biotechnology, 2023, 43, 124-132 [in Ukrainian]. https://doi.org/10.31073/vet_biotech43-12

MELNYCHUK V.1,2, e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., KOVALENKO S.1, e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.

1Poltava State Agrarian University
2Institute of Veterinary Medicine of the NAAS

 

COMPARATIVE EFFICIENCY OF CHORIOPTES BOVIS MITES MICROPREPARATIONS PREPARING

Introduction. Acariform parasitic mites belonging to the genus Chorioptes (Acariformes: Psoroptidae) are distributed worldwide, causing chorioptosis in many domestic and wild animals. The diagnosis of chorioptosis in ruminants is based on clinical signs and microscopic detection of mites in scrapings from the skin of the affected animal with mandatory species identification of choriopteses. Genetic diagnosis of choriopteses is expensive and requires quite a lot of time to carry it out, so it is necessary to search for new effective methods of diagnosing chorioptosis.

The goal of the work was to establish the effectiveness of the methods of preparing micropreparations from Chorioptes bovis in a comparative aspect.

Materials and methods. At the first stage of research, the most optimal values of the ratio proposed by us were determined: glycerin and alcohol solutions of iodine (1:1, 2:1, 3:1), which were used as a dye for the production of micropreparations from C. bovis.

At the second stage of research, the effectiveness of the methods of micropreparations preparing were compared, namely: it was proposed the one using an iodine-glycerol mixture and the well-known one using1% alcoholic solution of diamond green as a dye.

Results of research and discussion. The conducted studies established that the most effective method of preparing micropreparations from Chorioptes bovis in terms of the degree of staining of their morphological structures is the method using a mixture of glycerol and an alcohol solution of iodine (in a ratio of 3:1) compared to the method where a 1% alcohol solution of diamond green is used as a dye.

Conclusions and prospects for further research:

1. The most effective method of preparing micropreparations from Chorioptes bovis is the method using a mixture of glycerin and an alcohol solution of iodine in a ratio of 3:1.

2. When comparing the degree of staining of the morphological structures of choriopteses, the method using the iodine-glycerol mixture showed a high degree of staining according to 7 indicators, and the method using a 1% alcohol solution of diamond green – only according to 3 indicators.

The results obtained by us regarding the effectiveness of the methods of preparing micropreparations from choriopteses will be used in the following tests to establish the identification features of C. bovis.

Keywords: choriopotosis, cattle, diagnosis, temporary micropreparations, effectiveness.

REFERENCES

  1. Yeruham, I., Rosen, S., & Hadani, A. (1999). Chorioptic mange (Acarina: Psoroptidae) in domestic and wild ruminants in Israel. Experimental & Applied Acarology, 23(11), 861-869. https://doi.org/10.1023/a:1006217016688.
  2. Lusat, J., Bornstein, S., & Wall, R. (2011). Chorioptes mites: re-evaluation of species integrity. Medical and Veterinary Entomology, 25(4), 370-376. https://doi.org/10.1111/j.1365-2915.2011.00951.x.
  3. Wang, T., Xie, Y., Zheng, Y., Wang, C., Li, D., Koehler, A.V., & Gasser, R.B. (2018). Parasites of the Giant Panda: a risk factor in the conservation of a species. Advances in Parasitology, 99, 1-33. https://doi.org/10.1016/bs.apar.2017.12.003.
  4. Cremers, H.J. (1985). Chorioptes bovis (Acarina: Psoroptidae) in some camelids from Dutch zoos. Veterinary Quarterly, 7(3), 198-199. https://doi.org/10.1080/01652176.1985.9693983.
  5. He, R., Gu, X.B., Xie, Y., Peng, X.R., Angel, C., & Yang, G.Y. (2019). Transcriptome-based analysis of putative allergens of Chorioptes texanus. Parasites & Vectors, 12(1), 587. https://doi.org/10.1186/s13071-019-3843-7.
  6. Rehbein, S., Winter, R., Visser, M., Maciel, A.E., & Marley, S.E. (2005). Chorioptic mange in dairy cattle: treatment with eprinomectin pour-on. Parasitology Research, 98(1), 21-25. https://doi.org/10.1007/s00436-005-0005-y.
  7. Villarroel, A., & Halliburton, M.K. (2013). Control of extensive chorioptic mange natural infection in lactating dairy cattle without milk withdrawal. Veterinary Journal, 197(2), 233-237. https://doi.org/10.1016/j.tvjl.2013.01.003
  8. Yasine, A., Kumsa, B., Hailu, Y., & Ayana, D. (2015). Mites of sheep and goats in Oromia Zone of Amhara Region, North Eastern Ethiopia: species, prevalence and farmers awareness. BMC Veterinary Research, 11, 122. https://doi.org/10.1186/s12917-015-0433-6.
  9. Seid, K., Amare, S., & Tolossa, Y.H. (2016). Mange mites of sheep and goats in selected sites of Eastern Amhara region, Ethiopia. Journal of Parasitic Diseases, 40(1), 132-137. https://doi.org/10.1007/s12639-014-0463-1.
  10. Hestvik, G., Zahler-Rinder, M., Gavier-Widén, D., Lindberg, R., Mattsson, R., Morrison, D., & Bornstein, S. (2007). A previously unidentified Chorioptes species infesting outer ear canals of moose (Alces alces): characterization of the mite and the pathology of infestation. Acta Veterinaria Scandinavica, 49(1), 21. https://doi.org/10.1186/1751-0147-49-21.
  11. Suh, G.H., Hur, T.Y., Lim, S., Shin, S.M., Kwon, J., Cho, S.H., Lee, C.Y., & Shin, S.S. (2008). The first outbreak of Chorioptes texanus (Acari: Psoroptidae) infestation in a cattle farm in Korea. Korean Journal of Parasitology, 46(4), 273-278. https://doi.org/10.3347/kjp.2008.46.4.273.
  12. Vieira, M.I., Bordin, T., Dall'Agnol, B., Zanchin, F., Motta, A. C., & Noro, M. (2014). Re-emergence of Chorioptes bovis (Acari: Psoroptidae) in cattle in the state of Rio Grande do Sul, Brazil. Brazilian Journal of Veterinary Parasitology, 23(4), 530-533. https://doi.org/10.1590/S1984-29612014090.
  13. Marcondes, C.B., & Dantas-Torres, F. (2017). Diseases caused by Acari (ticks and mites). Arthropod Borne Diseases, 537-48. https://doi.org/10.1007/978-3-319-13884-8_34.
  14. Nematollahi A., Moghaddam G.A., & Golezardy H. (2007). An outbreak of Chorioptes bovis mange on a dairy farm in Tabriz, Iran. Iranian Journal of Veterinary Research, 8(4), 351-354.
  15. Zahler, M., Hendrikx, W.M., Essig, A., Rinder, H., & Gothe, R. (2001). Taxonomic reconsideration of the genus Chorioptes Gervais and van Beneden, 1859 (Acari: Psoroptidae). Experimental & Applied Acarology, 25(6), 517-523. https://doi.org/10.1023/a:1011857802317.
  16. Zahler, M., Hendrikx, W.M., Essig, A., Rinder, H., & Gothe, R. (2000). Species of the genus Psoroptes (Acari: Psoroptidae): a taxonomic consideration. Experimental & Applied Acarology, 24(3), 213-225. https://doi.org/10.1023/a:1006443722815.
  17. Losson, B.J., Mignon, B., Bossaert, K., Leclipteux, T., & Lonneux, J.F. (1998). Field efficacy of injectable doramectin against Chorioptes bovis in naturally infected cattle. Veterinary Record, 142(1), 18-19. https://doi.org/10.1136/vr.142.1.18.
  18. Dovhii, Yu.Yu., Feshchenko, D.V., Koriachkov, V.A., Zghozinska, O.A., Bakhur, T.I., Drahalchuk, A.I., & Stakhivskyi, O.V. (2011). Patent na korysnu model № 66145. Ukraina. Sposib koprolohichnoi diahnostyky helmintoziv i eimerioziv [Method for coprological diagnostics of helminthisms and eimerioses]. 
  19. Kovalenko, S., & Melnychuk, V.(2022). Efficiency of the proposed method of manufacturing temporary micropreparations of ticks of the genus Chorioptes. Bulletin of Poltava State Agrarian Academy, (4), 119-125. https://doi.org/10.31210/visnyk2022.04.14.
  20. Yevstafieva, V.O., Klymenko, O.S., & Khyzhnia, L.Yu. (2013). Patent na korysnu model №  85028. Ukraina. Sposib pryhotuvannia zbudnykiv riadu Mallophaga in toto [Method for the preparation of pathogens of the line Mallophaga in toto]. https://sis.nipo.gov.ua/uk/search/detail/1134435/ [in Ukrainian].
  21. Plant, J.D., Kutzler, M.A., & Cebra, C.K. (2007). Efficacy of topical eprinomectin in the treatment of Chorioptes sp. infestation in alpacas and llamas. Veterinary Dermatology, 18(1), 59-62. https://doi.org/10.1111/j.1365-3164.2007.00558.x.
  22. Rüfenacht, S., Roosje, P.J., Sager, H., Doherr, M.G., Straub, R., Goldinger-Müller, P., & Gerber, V. (2011). Combined moxidectin and environmental therapy do not eliminate Chorioptes bovis infestation in heavily feathered horses. Veterinary Dermatology, 22(1), 17-23. https://doi.org/10.1111/j.1365-3164.2010.00892.x.

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