Bulletin "Veterinary biotechnology"

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Veterynarna biotekhnolohiia – Veterinary biotechnology, 2023, 43, 166-174 [in Ukrainian]. https://doi.org/10.31073/vet_biotech43-16

TUYAKHOV M.F., email: This email address is being protected from spambots. You need JavaScript enabled to view it.

National university of life and environmental sciences of Ukraine

 

INVESTIGATION OF THE SENSITIVITY OF STAPHYLOCO

Introduction. Otitis externa is the most common ear disease in dogs, affecting up to 20% of the dog population. This disease is polyetiological in nature, but it is mainly a microbial infection.

Malassezia pachydermatis, as well as representatives of the genera Staphylococcus, Pseudomonas, Escherichia and Proteus are the most typical causative agents most often isolated from dogs with otitis externa and/or pyoderma.

The goal of the work. Determination of sensitivity of staphylococcal isolates for otitis in dogs for further rational planning of antibiotic therapy and prevention of selection of resistant strains of microorganisms.

Materials and methods. Experimental animals were dogs from 6 months to 10 years. During the period from December to September 2020, 20 dogs of various breeds weighing 5–55 kg of both sexes with otitis symptoms were examined.

Determination of the sensitivity of isolates to antibiotics and antifungal drugs was carried out by the method of diffusion in agar (disc method).

Results of research and discussion. As the research results showed, Staphylococcus aureus – 54.1% and Staphylococcus pseudintermedius (29.2%) prevailed among the microorganisms of the coccal group.

Among methicillin-resistant isolates of S. aureus, high sensitivity was observed only to vancomycin, ofloxacin, gentamicin and marbofloxacin. Isolates of S. pseudintermedius were sensitive to first-generation cephalosporins – cefazolin, trimethoprim-sulfamethoxazole, ciprofloxacin, ofloxacin, and lesser extent – to norfloxacin.

Representatives of S. epidermidis were highly resistant to most of the tested antibiotics. Thus, only vancomycin, imipenem, ofloxacin, meropenem, ciprofloxacin and norfloxacin significantly inhibited the growth of the pathogen. The isolates were slightly sensitive or resistant to other antibiotics.

Conclusions and prospects for further research. Based on the obtained data, it is planned to investigate the sensitivity of microbial isolates of the skin to antibiotics, especially microorganisms of cocci group, with further improvement of antibiotic therapy of animals affected with otitis, especially with the prevalence of methicillin-resistant staphylococci.

Keywords: dogs, microbiological research, nutrient media, skin microflora, microbial isolates, otitis, staphylococci, antibiotics sensitivity.

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