Bulletin "Veterinary biotechnology"

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Cite: KoshevoyV. I., Syniahovska K. A., Naumenko S. V., Radzykhovskyi M. L. Patohenetychne znachennia oksydatyvnoho stresu za hiperplazii peredmikhurovoi zalozy u psiv. [Pathogenetic significance of oxidative stress in canine prostatic hyperplasia]. Veterynarna biotekhnolohiia – Veterinary biotechnology, 46, 83-96. https://doi.org/10.31073/vet_biotech46-06 [in Ukrainian].

 

Koshevoy V. I. 1, e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.

SyniahovskaK. A. 1, e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.

Naumenko S.V. 1, e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.

Radzykhovskyi M. L. 2, e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.

1 State Biotechnological University

2 National University of Life Resources and Environmental Sciences of Ukraine

 

PATHOGENETIC SIGNIFICANCE OF OXIDATIVE STRESS IN CANINE PROSTATIC HYPERPLASIA

Introduction. Canine prostatic hyperplasia is a common disease, mainly in older males, which is registered in animals regardless of breed, fatness and other factors. The pathogenesis of this disease in dogs is not well studied. One of its components – hormonal changes, has been studied in more depth, as a result of which it is known that the development of prostatic hyperplasia occurs due to impaired androgenesis, changes in the testosterone-estradiol ratio. On the contrary, data on the redox balance in prostate tissue during hyperplastic changes are scarce.

The purpose of the work was to summarize and analyze data regarding the pathogenetic role of oxidative stress in prostatic hyperplasia in male dogs.

Research materials and methods. The study was carried out using methods of searching, processing, analyzing and summarizing data from professional scientific literature on the etiology, pathogenesis, pathophysiology and pathomorphology of prostate hyperplasia in dogs. Scientific articles from the international scientometric databases Scopus, Pubmed and Google Scholar published in 2000-2025 were used for the study.

Research results and their discussion. It has been established that in dogs with benign prostatic hyperplasia there is an increase in the intensity of lipoperoxidation processes under the influence of endogenous factors, which leads to oxidative stress, which results in a deficiency of antioxidants. Disturbances of redox processes play an important role in the mechanisms of development of prostatic hyperplasia in dogs and require correction.

Conclusions and prospects for further research. Prostatic hyperplasia in dogs is a common disease with a complex pathogenesis with interrelated changes in two systems – hormonally mediated mechanisms focused on changes in dihydrotestosterone levels, age-related increases in estrogen levels, and the action of prolactin; and imbalance in the prooxidant-antioxidant system caused by the accumulation of oxygen radicals that cause oxidative stress and a decrease in the protective activity of the antioxidant system. The perspective of further research a development of new anti-inflammatory agents for the treatment of prostate hyperplasia in dogs using nanostructured materials – nanoparticles of noble metals and rare earth elements.

Keywords: dogs, prostate, pathology, oxidative stress.

REFERENCES

  1. Naumenko, S., & Koshevoy, V. (2018). Disorder of andrological pathology in the eastern, southern and central regions of Ukraine for 2012-2017 (research data). Veterinary Science, Technologies of Animal Husbandry and Nature Management, (1), 86-88. Retrieved from http://ojs.hdzva.edu.ua/index.php/journal/article/view/80.
  2. Cho, S. E., Fudge, J. M., Kim, S., Page, B., & Yu, D. (2025). Cryptorchidism in dogs and cats presented for elective gonadectomy: A descriptive cohort study of 306 animals treated between 2018 and 2023. Topics in companion animal medicine, 65, 100961. https://doi.org/10.1016/j.tcam.2025.100961.
  3. Aires, L. P. N., Gasser, B., Del'Aguila-Silva, P., Gonçalves, J. D., Uscategui, R. A. R., Spada, S., De Felice, D., Russo, M., Lima, B. B., Gimenes, L. U., & Feliciano, M. A. R. (2025). Multiparametric ultrasound (MPUS) evaluation of the testes of normozoospermic dogs - a pilot study. Scientific reports, 15(1), 7121. https://doi.org/10.1038/s41598-025-91087-9.
  4. Koshevoy, V. I., Naumenko, S. V., Klochkov, V. K., & Yefimova, S. L. (2021). The use of gadolinium orthovanadate nanoparticles for the correction of reproductive ability in boars under oxidative stress. Ukrainian Journal of Veterinary Sciences, 12(2), 74–82. https://doi.org/10.31548/ujvs.2021.02.008.
  5. Ciaputa, R., Brambilla, E., Dzimira, S., Nowak, M., Janus-Ziółkowska, I., Piotrowska, A., Tomaszek, A., Kandefer-Gola, M., & Grieco, V. (2025). Immunohistochemical expression of testin protein in testicular tumours in dogs. Theriogenology, 239, 117375. https://doi.org/10.1016/j.theriogenology.2025.117375.
  6. Pritchard, G. C., Scholes, S. F., Foster, A. P., Mitchell, E. S., Lawes, J., Ibata, G., & Banks, M. (2008). Ulcerative vulvitis and balanitis in sheep flocks. The Veterinary record, 163(3), 86–89. https://doi.org/10.1136/vr.163.3.86.
  7. Falchi, L., Pau, S., Ledda, M., Melosu, V., & Zedda, M. T. (2023). Lesions of the prepuce and penis in rams: A retrospective study. Veterinary research communications, 47(4), 2259–2264. https://doi.org/10.1007/s11259-023-10128-8.
  8. Pinheiro, D., Machado, J., Viegas, C., Baptista, C., Bastos, E., Magalhães, J., Pires, M. A., Cardoso, L., & Martins-Bessa, A. (2017). Evaluation of biomarker canine-prostate specific arginine esterase (CPSE) for the diagnosis of benign prostatic hyperplasia. BMC veterinary research, 13(1), 76. https://doi.org/10.1186/s12917-017-0996-5.
  9. Serhiienko, V., Koshevoy, V., Naumenko, S., Kotyk, B., Ilina, O., Shchepetilnikov, Y., Makhotina, D., & Marakhovskyi, I. (2025). Oxidative Stress Markers, Antioxidant Balance, and Protein Metabolism in Dogs with Acute Prostatitis. World Veterinary Journal, 15(1), 167–175. https://www.doi.org/10.54203/scil.2025.wvj19.
  10. Memon M. A. (2007). Common causes of male dog infertility. Theriogenology, 68(3), 322–328. https://doi.org/10.1016/j.theriogenology.2007.04.025.
  11. Laurusevičius, T., Šiugždaitė, J., Juodžiukynienė, N., Kerzienė, S., Anskienė, L., Jackutė, V., Trumbeckas, D., Van Soom, A., Posastiuc, F. P., & Žilinskas, H. (2024). Comparative Evaluation of Diagnostic Methods for Subclinical Benign Prostatic Hyperplasia in Intact Breeding Male Dogs. Animals : an open access journal from MDPI, 14(8), 1204. https://doi.org/10.3390/ani14081204.
  12. Domoslawska, A., Zduńczyk, S., Kankofer, M., & Bielecka, A. (2022). Oxidative stress biomarkers in dogs with benign prostatic hyperplasia. Irish veterinary journal, 75(1), article number 21. https://doi.org/10.1186/s13620-022-00228-3.
  13. Ponglowhapan, S., Pattanaviboon, K., Maneerattanavichien, Y., Phitchayangkoon, P., Lueangprasert, P., Preechatangkit, A., Suthigarn, S., Saisawart, P., Thanaboonnipat, C., & Choisunirachon, N. (2024). Ultrasonographic measurement of the prostatic dimension-to-aortic luminal diameter in healthy intact male dogs and dogs with benign prostatic hyperplasia. The Journal of small animal practice, 65(11), 789–798. https://doi.org/10.1111/jsap.13766.
  14. Domosławska, A., & Zdunczyk, S. (2020). Clinical and spermatological findings in male dogs with acquired infertility: A retrospective analysis. Andrologia, 52(11), e13802. https://doi.org/10.1111/and.13802.
  15. Ruetten, H., Wehber, M., Murphy, M., Cole, C., Sandhu, S., Oakes, S., Bjorling, D., Waller, K., 3rd, Viviano, K., & Vezina, C. (2021). A retrospective review of canine benign prostatic hyperplasia with and without prostatitis. Clinical theriogenology, 13(4), 360–366.
  16. Cazzuli, G., Damián, J. P., Minini, L., Ferreira, F., Suárez, G., & Pessina, P. (2025). Use of Epilobium parviflorum in the Treatment of Benign Prostatic Hyperplasia in Canines. Veterinary medicine and science, 11(2), e70205. https://doi.org/10.1002/vms3.70205.
  17. Domosławska, A., Zduńczyk, S., Bielecka, A., & Kankofer, M. (2023). The effect of benign prostatic hyperplasia on total antioxidant capacity and protein peroxidation in canine prostatic fluid and spermatozoa. Polish journal of veterinary sciences, 26(4), 667–673. https://doi.org/10.24425/pjvs.2023.148286.
  18. Sun, J., Sherryn, S., Tong, Z., Xia, S., & Chen, B. (2024). Evaluation of safety and efficacy of insulated-gate bipolar transistor holmium laser technology for prostate enucleation in canine model: a preliminary study. Lasers in medical science, 39(1), 201. https://doi.org/10.1007/s10103-024-04155-3.
  19. Sun, F., Báez-Díaz, C., & Sánchez-Margallo, F. M. (2017). Canine prostate models in preclinical studies of minimally invasive interventions: part II, benign prostatic hyperplasia models. Translational andrology and urology, 6(3), 547–555. https://doi.org/10.21037/tau.2017.03.62.
  20. Tan, W. X., Khor, K. H., & Chan, S. M. (2015). Cystic benign prostatic hyperplasia in a dog. Jurnal Veterinar Malaysia, 27(1), 16–19.
  21. Pasikowska, J., Hebel, M., Niżański, W., & Nowak, M. (2015). Computed Tomography of the Prostate Gland in Healthy Intact Dogs and Dogs with Benign Prostatic Hyperplasia. Reproduction in domestic animals = Zuchthygiene, 50(5), 776–783. https://doi.org/10.1111/rda.12587.
  22. Cai, Z., Li, H., Dong, X., Wei, J., Zhang, J., Zhang, Y., Huang, L., Zhu, J., & Liu, Z. (2024). Effect of Acoustically Responsive Droplet-based Low-intensity Histotripsy on Canine Prostate. Ultrasound in medicine & biology, 50(12), 1955–1963. https://doi.org/10.1016/j.ultrasmedbio.2024.09.001.
  23. Leav, I., Schelling, K. H., Adams, J. Y., Merk, F. B., & Alroy, J. (2001). Role of canine basal cells in postnatal prostatic development, induction of hyperplasia, and sex hormone-stimulated growth; and the ductal origin of carcinoma. The Prostate, 48(3), 210–224. https://doi.org/10.1002/pros.1100.
  24. Xia, Y., Yuan, T., Zou, W., Li, H., Ning, J., Ruan, Y., Xu, L., Yu, W., & Cheng, F. (2023). Biocompatibility and efficacy of prostatic urethral lift in benign prostate hyperplasia: an in vivo and in vitro study. Scientific reports, 13(1), 13828. https://doi.org/10.1038/s41598-023-40889-w.
  25. Ferré-Dolcet, L., Pisu, M. C., Attard, S., & Romagnoli, S. (2023). Effect of the GnRH stimulation test on Canine Prostatic-Specific Esterase (CPSE). Reproduction in domestic animals = Zuchthygiene, 58(8), 1125–1131. https://doi.org/10.1111/rda.14413.
  26. Domosławska-Wyderska, A., Zduńczyk, S., & Rafalska, A. (2024). Potential role of oxidative stress in pathogenesis of benign prostatic hyperplasia in male dogs. Reproduction in domestic animals = Zuchthygiene, 59(5), e14580. https://doi.org/10.1111/rda.14580.
  27. Holst, B. S., & Nilsson, S. (2023). Age, weight and circulating concentrations of total testosterone are associated with the relative prostatic size in adult intact male dogs. Theriogenology, 198, 356–360. https://doi.org/10.1016/j.theriogenology.2022.12.047.
  28. Wang, K., Huang, D., Zhou, P., Su, X., Yang, R., Shao, C., & Wu, J. (2022). Bisphenol A exposure triggers the malignant transformation of prostatic hyperplasia in beagle dogs via cfa-miR-204/KRAS axis. Ecotoxicology and environmental safety, 235, 113430. https://doi.org/10.1016/j.ecoenv.2022.113430.
  29. Smith, J. (2008). Canine prostatic disease: a review of anatomy, pathology, diagnosis, and treatment. Theriogenology, 70(3), 375–383. https://doi.org/10.1016/j.theriogenology.2008.04.039.
  30. Cunto, M., Mariani, E., Anicito Guido, E., Ballotta, G., & Zambelli, D. (2019). Clinical approach to prostatic diseases in the dog. Reproduction in domestic animals = Zuchthygiene, 54(6), 815–822. https://doi.org/10.1111/rda.13437.
  31. Tezuka, M., Yonekubo-Awaka, S., Tamai, Y., Tsuchioka, K., Kobayashi, K., Kuramochi, Y., Tatemichi, S., Nagasawa, T., & Kiguchi, S. (2023). Suppression of hypothalamic-pituitary-gonadal function by linzagolix in benign prostatic hyperplasia and polycystic ovary syndrome animal models. Clinical and experimental pharmacology & physiology, 50(11), 914–923. https://doi.org/10.1111/1440-1681.13817.
  32. Lima, C. B., Angrimani, D. S. R., Flores, R. B., & Vannucchi, C. I. (2021). Endocrine, prostatic vascular, and proapoptotic changes in dogs with benign prostatic hyperplasia treated medically or surgically. Domestic animal endocrinology, 75, 106601. https://doi.org/10.1016/j.domaniend.2020.106601.
  33. Banerjee, P. P., Banerjee, S., Brown, T. R., & Zirkin, B. R. (2018). Androgen action in prostate function and disease. American journal of clinical and experimental urology, 6(2), 62–77.
  34. Angrimani, D. S. R., Francischini, M. C. P., Brito, M. M., & Vannucchi, C. I. (2020). Prostatic hyperplasia: Vascularization, hemodynamic and hormonal analysis of dogs treated with finasteride or orchiectomy. PloS one, 15(6), e0234714. https://doi.org/10.1371/journal.pone.0234714.
  35. Banerjee, P. P., Banerjee, S., & Brown, T. R. (2001). Increased androgen receptor expression correlates with development of age-dependent, lobe-specific spontaneous hyperplasia of the brown Norway rat prostate. Endocrinology, 142(9), 4066–4075. https://doi.org/10.1210/endo.142.9.8376.
  36. Shafi, A. A., Yen, A. E., & Weigel, N. L. (2013). Androgen receptors in hormone-dependent and castration-resistant prostate cancer. Pharmacology & therapeutics, 140(3), 223–238. https://doi.org/10.1016/j.pharmthera.2013.07.003.
  37. Tang, X. H., Liu, Z. Y., Ren, J. W., Zhang, H., Tian, Y., Hu, J. X., Sun, Z. L., & Luo, G. H. (2025). Comprehensive RNA-seq analysis of benign prostatic hyperplasia (BPH) in rats exposed to testosterone and estradiol. Scientific reports, 15(1), 2750. https://doi.org/10.1038/s41598-025-87205-2.
  38. Lai, K. P., Huang, C. K., Fang, L. Y., Izumi, K., Lo, C. W., Wood, R., Kindblom, J., Yeh, S., & Chang, C. (2013). Targeting stromal androgen receptor suppresses prolactin-driven benign prostatic hyperplasia (BPH). Molecular endocrinology (Baltimore, Md.), 27(10), 1617–1631. https://doi.org/10.1210/me.2013-1207.
  39. Pettina, G., Bucci, R., Mazzetti, A., Quartuccio, M., Robbe, D., & Pisu, M. C. (2024). Contrast-Enhanced Ultrasound (CEUS) Evaluation of Canine Prostatic Hyperplasia before and after Osaterone Acetate Therapy: Preliminary Results. Animals : an open access journal from MDPI, 14(11), 1683. https://doi.org/10.3390/ani14111683.
  40. Minciullo, P. L., Inferrera, A., Navarra, M., Calapai, G., Magno, C., & Gangemi, S. (2015). Oxidative stress in benign prostatic hyperplasia: a systematic review. Urologia internationalis, 94(3), 249–254. https://doi.org/10.1159/000366210.
  41. Koshevoy, V., Naumenko, S., Skliarov, P., Fedorenko, S., & Kostyshyn, L. (2021). Male infertility: Pathogenetic significance of oxidative stress and antioxidant defence (review). Scientific Horizons, 24(6), 107–116. https://www.doi.org/10.48077/scihor.24(6).2021.107-116.
  42. Liu, H., Zhou, Y., Wang, Z., Liu, D., Li, Y., Lai, H., Qiu, J., Shan, S., Guo, F., Chen, P., Guo, Y., Zeng, G., DiSanto, M. E., & Zhang, X. (2025). Heat Shock Protein Family A Member 1A Attenuates Apoptosis and Oxidative Stress via ERK/JNK Pathway in Hyperplastic Prostate. MedComm, 6(3), e70129. https://doi.org/10.1002/mco2.70129.
  43. Bostanci, Y., Kazzazi, A., Momtahen, S., Laze, J., & Djavan, B. (2013). Correlation between benign prostatic hyperplasia and inflammation. Current opinion in urology, 23(1), 5–10. https://doi.org/10.1097/MOU.0b013e32835abd4a.
  44. Tong, Y., & Zhou, R. Y. (2020). Review of the Roles and Interaction of Androgen and Inflammation in Benign Prostatic Hyperplasia. Mediators of inflammation, 2020, article number 7958316. https://doi.org/10.1155/2020/7958316.
  45. Vital, P., Castro, P., & Ittmann, M. (2016). Oxidative stress promotes benign prostatic hyperplasia. The Prostate, 76(1), 58–67. https://doi.org/10.1002/pros.23100.
  46. Roumeguère, T., Sfeir, J., El Rassy, E., Albisinni, S., Van Antwerpen, P., Boudjeltia, K. Z., Farès, N., Kattan, J., & Aoun, F. (2017). Oxidative stress and prostatic diseases. Molecular and clinical oncology, 7(5), 723–728. https://doi.org/10.3892/mco.2017.1413.
  47. Gandaglia, G., Briganti, A., Gontero, P., Mondaini, N., Novara, G., Salonia, A., Sciarra, A., & Montorsi, F. (2013). The role of chronic prostatic inflammation in the pathogenesis and progression of benign prostatic hyperplasia (BPH). BJU international, 112(4), 432–441. https://doi.org/10.1111/bju.12118.
  48. Kaltsas, A., Giannakas, T., Stavropoulos, M., Kratiras, Z., & Chrisofos, M. (2025). Oxidative Stress in Benign Prostatic Hyperplasia: Mechanisms, Clinical Relevance and Therapeutic Perspectives. Diseases (Basel, Switzerland), 13(2), 53. https://doi.org/10.3390/diseases13020053.
  49. Angrimani, D. S. R., Brito, M. M., Rui, B. R., Nichi, M., & Vannucchi, C. I. (2020). Reproductive and endocrinological effects of Benign Prostatic Hyperplasia and finasteride therapy in dogs. Scientific reports, 10(1), article number 14834. https://doi.org/10.1038/s41598-020-71691-7.
  50. Dearakhshandeh, N., Mogheiseh, A., Nazifi, S., Ahrari Khafi, M. S., Abbaszadeh Hasiri, M., & Golchin-Rad, K. (2019). Changes in the oxidative stress factors and inflammatory proteins following the treatment of BPH-induced dogs with an anti-proliferative agent called tadalafil. Journal of veterinary pharmacology and therapeutics, 42(6), 665–672. https://doi.org/10.1111/jvp.12805.
  51. Domoslawska-Wyderska, A., Orzołek, A., Zduńczyk, S., & Rafalska, A. (2023). Nitric oxide production by spermatozoa and sperm characteristics in dogs with benign prostatic hyperplasia. Polish journal of veterinary sciences, 26(4), 621–628. https://doi.org/10.24425/pjvs.2023.148281.
  52. Domoslawska, A., Zdunczyk, S., Franczyk, M., Kankofer, M., & Janowski, T. (2019). Total antioxidant capacity and protein peroxidation intensity in seminal plasma of infertile and fertile dogs. Reproduction in domestic animals = Zuchthygiene, 54(2), 252–257. https://doi.org/10.1111/rda.13345.
  53. Niżański, W., Levy, X., Ochota, M., & Pasikowska, J. (2014). Pharmacological treatment for common prostatic conditions in dogs - benign prostatic hyperplasia and prostatitis: an update. Reproduction in domestic animals = Zuchthygiene, 49(2), 8–15. https://doi.org/10.1111/rda.12297.
  54. Ploypetch, S., Wongbandue, G., Roytrakul, S., Phaonakrop, N., & Prapaiwan, N. (2023). Comparative Serum Proteome Profiling of Canine Benign Prostatic Hyperplasia before and after Castration. Animals: an open access journal from MDPI, 13(24), 3853. https://doi.org/10.3390/ani13243853.
  55. Koshevoy, V., Naumenko, S., Skliarov, P., Syniahovska, K., Vikulina, G., Klochkov, V., & Yefimova, S. (2022). Effect of gadolinium orthovanadate nanoparticles on male rabbits’ reproductive performance under oxidative stress. World’s Veterinary Journal, 12(3), 296–303. https://www.doi.org/10.54203/scil.2022.wvj37.
  56. Naumenko, S., Koshevoy, V., Matsenko, O., Miroshnikova, O., Zhukova, I., & Bespalova, I. (2023). Antioxidant properties and toxic risks of using metal nanoparticles on health and productivity in poultry. Journal of World`s Poultry Research, 13(3), 292–306. https://www.doi.org/10.36380/jwpr.2023.32.

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