Bulletin "Veterinary biotechnology"

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Cite: Kolchyk O.V., Akimov O.V. Rezultaty obrobky kontaminovanoi spermy protymikrobnymy substantsiiamy dlia pidvyshchennia rivnia biobezpeky u svynarstvi. [Results of treatment contaminated sperm with anti-microbal substances for advanced biosafety in pigs]Veterynarna biotekhnolohiia – Veterinary biotechnology, 46, 124-133. https://doi.org/10.31073/vet_biotech46-08 [in Ukrainian].

 

Kolchyk O.V., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.

AkimovO.V., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.

National Scientific Center "Institute of Experimental and Clinical Veterinary Medicine", Kharkiv, Ukraine

 

RESULTS OF TREATMENT CONTAMINATED SPERM WITH ANTI-MICROBIAL SUBSTANCES FOR ADVANCED BIOSAFETY IN PIGS

Introduction. One of the important aspects of assessing skewness is microbial contamination of the ejaculate, which can not only reduce sperm production, but also prevent increased infections and illnesses among animals.

The goal of the work was the significance of the antimicrobial activity of 2 antimicrobial substances Amiksin and Metisazon on Neisseria gonorrhoeae on biofuel-inducing microorganisms seen from the sperm of cows.

Materials and methods. The sows were clinically healthy, and metritis was recorded in the sows, which was manifested by infections of the ovarian organs and ovaries. From 5 boars (2-3) – in total, 12 sperm samples were collected. After collecting sperm samples, clear indicators and microbial contamination were assessed.

Results of research and discussion. The association of bacteria Neisseria gonorrhoeae, Pasteurella multocida, Pseudomonas aeruginosa, Enterobacter aerogenes was observed from sperm and showed multi-resistance to antimicrobial drug. At the early stage of research, a dilution of the composite Amiksin and Metisason was selected to improve the sperm of the dogs from bacterial contamination. The bactericidal effect of the composite was observed in a dilution of 10-3 for Neisseria gonorrhoeae, Pasteurella multocida Enterobacter aerogenes and Pseudomonas aeruginosa - 10-2, which was confirmed by the constant growth of microorganisms at replanting on large crops

Conclusions and prospects for further research. Antimicrobial substances Amiksin and Methisazone exhibit synergistic bactericidal activity against aquatic microorganisms in dilutions of 10-2 – 10-3 against pathogenic microflora in sperm of boars, which is promising for the development of sperm growth.

Keywords: sperm, boars, microbial contamination, biofuel-cleaning bacteria, bactericidal activity.

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