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Citation: Derkach S., Zhuk Y., Kovpak V., Valchuk O., Kharkevych Y. (2026) The reproductive potential of cows according to the concentration of anti-mueller hormone in their blood serum. Veterinary Biotechnology, 48, 32−49. https://doi.org/10.31073/vet_biotech48-02

DERKACH S., Cand. Sci. (Vet. Med.), Assoc. Prof., This email address is being protected from spambots. You need JavaScript enabled to view it., https://orcid.org/0000-0002-6174-1377 

ZHUK Y., Cand. Sci. (Vet. Med.), Assoc. Prof., This email address is being protected from spambots. You need JavaScript enabled to view it., https://orcid.org/0000-0002-8827

KOVPAK V., Cand. Sci., Prof. This email address is being protected from spambots. You need JavaScript enabled to view it., https://orcid.org/0000-0003-2419-1246

VALCHUK O., Cand. Sci. (Vet. Med.), Assoc. Prof., This email address is being protected from spambots. You need JavaScript enabled to view it. , https://orcid.org/0000-0002-4178-0352

KHARKEVYCH Y. Cand. Sci. (Vet. Med.), Assoc. Prof., This email address is being protected from spambots. You need JavaScript enabled to view it., https://orcid.org/0000-0002-7877-8272

THE REPRODUCTIVE POTENTIAL OF COWS ACCORDING TOTHE CONCENTRATION OF ANTI-MUELLER HORMONE IN THEIR BLOOD SERUM

Introduction. Anti-müllerian hormone at the initial stages of folliculogenesis induces changes in oocyte apoptosis and regulates the rate of primordial follicle formation. Anti-müllerian hormone gene expression in cattle is limited by granulosa cells of growing follicles, which makes this hormone a predictor of ovarian response to superovulation in cows. Its concentration in cow blood serum correlates with both the number of antral follicles and the number of ovulations. On the other hand, during the physiological estrous cycle, an increase in the concentration of endogenous follicle-stimulating hormone in cows blood is responsible for the recruitment of follicles in the ovary and their growth, while a subsequent decrease in the concentration of this hormone is responsible for the selection of one dominant follicle. The introduction of exogenous follicle-stimulating hormone stimulates the growth of recruited follicles, which allows a large number of follicles to acquire a dominant phenotype.

The goal of the work. To assess the reproductive potential of cows according to the concentration of anti-mueller hormone in their blood serum with external stimulation of folliculogenesis with different doses of follicle-stimulating hormone.

Materials and methods. The experiment was conducted on 30 Ukrainian black-and-white dairy cows after 1–2 lactations, in which the concentration of anti-müllerian hormone in the blood serum was estimated. Superovulation stimulation was performed in donor cows with different doses of follicle-stimulating hormone. Insemination of cows was performed twice. Embryos were washed out by a non-surgical method on the 7th day after the first insemination, after which they were counted and evaluated.

Results of research and discussion. During the study, a decrease in all studied parameters (number and quality of embryos) was noted when administering both lower and higher doses of follicle-stimulating hormone to cows than that recommended by the manufacturer of the drug regardless of serum concentration of anti-mueller hormone in cows.

Conclusions and prospects for further research. The most optimal dose of follicle-stimulating hormone to obtain the maximum number of high-quality embryos in groups of experimental animals is 1000 IU, which does not depend on the concentration of anti-müllerian hormone in their serum. The use of both lower (800 IU) and higher (1500) doses of follicle-stimulating hormone within individual experimental groups of animals leads to a decrease in the number of washed embryos in general and high-quality embryos in particular.

Further research will be aimed at determining the optimal parameters for selecting donor cows and determining the optimal superovulation scheme.

Keywords: donor cow, reproductive potential, hormonal stimulation, anti-mullerian hormone, follicle-stimulating hormone, embryo.

 

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