Veterynarna biotehnologija – Veterinary biotechnology, 2016, 29, 28-33 [in Ukrainian].

BABKIN M.V., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., GAVRASIEVA N.V., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., KYZMICH G.S., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.

State Scientific Control Institute of Biotechnology and Strains of Microorganisms

OBTAININIG OF PRIMARY CELL CULTURE FROM FRESHWATER MOLLUSCS

Introduction. Molluscs have the ability to regenerate, but they are not characteristic of malignant tumors. All attempts to get inoculated cell line cultures from the tissue of these animals were not successful. Perhaps the inability of the mollusk body cells segmentation in vitro and low «cancerous potential» causes failure to get finite cell lines.

The goal of the work. The objective of our work was to obtain primary cell cultures of freshwater bivalves, class bivalves (Bivalvia).

Materials and methods. In the study we used three methods enzymatic, mechanical and combination of both methods to obtain primary cell cultures. Observation of cell culture was performed using inverted microscope.

Results of research and discussion. Primary culture of freshwater mollusk cultured for 30 days at different temperatures and media. The optimum temperature for cultivation was +20 and +25C°, at a temperature 37C° mollusc cell migration was observed. The largest freshwater molluscs cells output was observed when using the combined method of obtaining primary culture cells. Also, it was observed by using a combination of two culture mediums DMEM and F-12 (1:1) +20% fetal bovine serum.

Conclusions and prospects for further research. There were showed results of obtaining of primary freshwater clam culture class bivalves (Bivalvia) by different methods. We used enzymatic, mechanical methods and combination of both methods as well. It was found that the largest freshwater molluscs cells yield was observed using the combined method of obtaining primary culture cells. Also, it was observed when using a combination of two media DMEM and F-12 (1:1) +20% fetal bovine serum. In addition, the optimal temperature +20 and + 25°C was chosen for primary culture culturing. The prospect of further research is to obtain passaged cell culture from freshwater molluscs. The obtained culture can be used in biotechnology, veterinary and humane medicine.

Keywords: freshwater molluscs, primary cell culture, nutrient media, culturing cell cultures.

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