Bulletin "Veterinary biotechnology"

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Veterynarna biotehnologija – Veterinary biotechnology, 2016, 29, 34-42 [in Ukrainian].

BABKINA M.M., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., TARASOV O.A., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.

Institute of Veterinary Medicine of the NAAS

PALCHYKOVSKA L.G., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., VASYLCHENKO O.V., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.

Institute of Molecular Biology and Genetics of the NAS of Ukraine

DERIABIN O.M., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.

State Scientific Control Institute of Biotechnology and Strains of Microorganisms

DETERMINATION OF ANTIBACTERIAL ACTIVITY OF TRHEECYCLIC TRIAZINES AGAINST KLEBSIELLA PNEUMONIAE

Introduction. Nowadays the important task for scientists is to intensify search for new compounds with antimicrobial activity of different chemical classes what can be used as a base for creating new antimicrobial drugs. The results suggest that a way to search the new perspective and approaches to the development of innovative synthetic drugs lies in the field of analysis of newly synthesized compounds.

The goal of the work was to study antibacterial properties of substances of threecyclic triazines class compounds towards Klebsiella pneumoniae.

Materials and methods. In this work it was used the substances of class of threecyclic triazines and Klebsiella pneumoniae pathogen. In the research disco-diffusion method and method of serial microdilution were used.

Results of research and discussion. It was detected that threecyclic triazines had sufficient activity against Klebsiella pneumoniae. The most active among the tested substances were compounds ODI-44, ODI-45, ODI-46, ODI-48 and ODI-57 with MIC 0.0041 mg/cm3 and growth inhibition zone from 23±0.36 mm to 24.6±0.21 mm.

Conclusion and prospects for further research: 1. It was revealed that chemical compounds of treecyclic triazines showed antimicrobial activity against Klebsiella pneumoniae. 2. The most promising compounds are ODI-44 (3-oxo-2,3-dihydro-1H-[1,2,4]triazino[5,6-b][1,4]benzothiazine-7-carboxylic acid), ODI-45 (7-methyl-3-oxo-2,3-dihydro-1H-[1,2,4]triazino[5,6-b][1,4]benzothiazine-9-carboxylic acid), ODI-46 (2,4-dioxo-1,3,4,5-tetrahydro-2H-pyrimido[4,5-b][1,4]benzothiazine-7-carboxylic acid), ODI-48 (3-(dimethylamino)-2H-[1,2,4]triazino[6,5-b][1,4]benzothiazine-8-carboxylic acid) and ODI-57(7-methyl-3-oxo-N-[4-(trifluoromethyl)phenyl]-2,3-dihydro-1H-[1,2,4]triazino[5,6-b][1,4]benzothiazine-9-carboxamide) and they can be considered as a candidates for future research.

Keywords: antibacterial activity, threecyclic triazines, Klebsiella pneumoniae, minimal inhibitory concentration, zones of growth inhibition.

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