Veterynarna biotehnologija – Veterinary biotechnology, 2016, 29, 75-83 [in Ukrainian].
GARKAVENKO T.A., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., AZYRKINA I.M., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., SHALIMOVA L.O., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.
State Scientific and Research Institute of Laboratory Diagnostics and Veterinary and Sanitary Expertise
DETERMINATION OF THE RESIDUAL AMOUNT OF MACROLIDES AND β-LACTAMS IN POULTRY PRODUCTS BY MICROBIOLOGICAL METHOD
Introduction. An urgent problem today remains the effective control of poultry products for the residual amounts of macrolides and β-lactams, as they able to get through the food chain to the human body, and cause overgrowth, toxemia, allergic reactions, secondary fungal infections, violation of mineral metabolism. They characterize as osteotropic and promote the development of antibiotic-resistant microorganisms in humans.
The goal of the work was to comparative the national and European microbiological screening methods for antibiotic residual in poultry meat and eggs; to conduct testing and validation of qualitative microbiological «NAT-screening» method of determination of macrolides and β-lactams in poultry, eggs and egg products.
Materialis and methods. Results are treated by variation statistics methods using «Microsoft Excel – 15.0» program with calculating the arithmetic mean (M), standard deviation (m) and the level of probability (p) by Student’s table. We determined the accuracy, specificity, sensitivity of method.
Results of research and discussion. We found clearly defined growth inhibition zone of pure test culture Kocuria rhizophila ATCC 9341, which ranged from 17.01±0.01 to 20.03±0.03 mm. The result of the study of microbiological “NAT-screening” method of small amounts of beta-lactams and macrolides residuals detection (½ MRL) showed that this index for meat was 25 g/kg, for eggs and egg products – 50 mg/kg. Specificity, accuracy and sensitivity of test was 100%.
Conclusions and prospects for further research. Established that sensitivity of European microbiological method «NAT-screening» for residual amounts of macrolides and β-lactams for meat were: tylosin – 100 µg/kg, lincomycin – 100 µg/kg, tiamulin – 100 µg/kg, spiramycin – 200 µg/kg, Tilmicosin – 75 µg/kg, erythromycin – 200 µg/kg, ampicillin – 50 µg/kg, amoxicillin – 50 µg/kg, cloxacillin – 300 µg/kg, dicloxacloxacillin – 300 µg/kg, oxacillin – 300 µg/kg; for eggs and egg products – erythromycin – 150 µg/kg, lincomycin – 50 µg/kg, tiamulin – 1000 µg/kg, tylosin – 200 µg/kg corresponding MDR European law and order №695 from 06.08.2013.
Keywords: test culture, Kocuria rhizophila АТСС 9341, macrolides, β-lactams, tylosin, poultry, eggs, egg products, NAT-screening.
REFERENCES
