Veterynarna biotehnologija – Veterinary biotechnology, 2016, 29, 132-137 [in Ukrainian].
KOVALENKO V.L., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.., VASYANOVICH O.M., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.
Institute of Veterinary Medicine of the NAAS
ZAGREBELNYI A.V., e-mail: kvl_с@mail.ru
State Scientific and Research Institute of Laboratory Diagnostics and Veterinary and Sanitary Expertise
STUDIES ON THE EFFECT OF DISINFECTANTS ON FUNGI SPECIES ASPERGILLUS, PENICILLIUM, FUSARIUM AND CANDIDA
Introduction. The success of the fight against infectious diseases and their prevention largely depends on the quality of the disinfection. One of the criteria for determining the quality of disinfection is the use of micromycetes or mould fungus involved in the pathogenesis of various diseases of humans and animals: fungal infections, mycetogenetic allergic diseases, allergic bronchopulmonary aspergillosis, allergic fungi rynosinusitis and others.
The goal of the work. To explore the impact of disinfectant “Orgasept” on the development of micromycetes. To determine probability of using the disinfectant application to inhibit the development of micromycetes on the surface of test objects.
Materials and methods. A disinfectant “Orgasept” was chosen as the object of the research. Chapek, medium, glucose agar Sabouraud were prepared in accordance with all recommendations, and used in the research. The authors used the specific strains of micromycetes, which are highly resistant to disinfectants. We used Aspergillus, Penicillium, Fusarium and Candida albicans micromycetes.
Results of research and discussion. Analysing the results, it should be noted that the recommended concentration of Orgasept 3% under 30 minute exposition was effective causing inhibition zone of fungi. Growth of micromycetes were not registered during the research. At the same time, 0.25% concentration of disinfectant showed positive results on Candida albicans.The results of the agar-diffuse and paper disks-based method showed that the higher was the disinfectant’s concentration, the larger inhibition zone emerged. The disinfectant showed a fungistatic effect regarding Aspergillus, Penicillium, Fusarium fungi.
Conclusions and prospects for further research. It was found that Orgasept showed a fungi static effect on Aspergillis, Penicillium and Fusarium fungi in all recommended concentrations (3% under 30–60 minutes of exposition) and in 0.25% concentration regarding Candida albicans. Under the paper disks-based and the agar-diffuse methods, the effective concentration of Orgasept was from 1% under 60 minutes exposition.
Keywords: disinfection, disinfectant, Orgasept, fungicide, micromycetes.
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