Veterynarna biotehnologija – Veterinary biotechnology, 2016, 29, 137-146 [in Ukrainian].

KOVPAK V.V., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., KHARKEVYCH I.O., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.

National University of Life and Environmental Sciences of Ukraine

GUDZ N.V., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.

Institute of Veterinary Medicine of the NAAS

IMMUNE STATUS OF RATS AT AN ALLOXAN DIABETES AFTER ADMINISTRATION OF CELL CULTURES

Introduction. Diabetes mellitus (DM) – a complex systemic disease caused by absolute or relative deficiency of the hormone insulin. Effective treatment of this disease does not exist. Therefore, the search and development of alternative methods of treatment of diabetes mellitus in animals are always in the field of view of scientists. One such method is the use of cells as a replacement therapy.

Goal of the work. Explore the levels of glucose, total and differential leucocytes in the peripheral blood of rats, some of their functional parameters, the content of circulating immune complexes and immunoglobulins in the background modeling alloxan diabetes in the intraperitoneal introduction of culture cells of bone marrow (CCBM) and culture cells of the pancreas (CCP).

Materials and methods. In the experiment used 15 rats. Rats were divided into 5 groups of 3 animals each: I – control (intact animals), II – experimental, without therapeutic interventions (blood sampling for analysis was performed on the 20th day of the experiment), III – experimental, without therapeutic intervention (selection of blood – 34 the day of the experiment), IV – experimental animals which on the 20th day after the formation of the DM entered the CCBM (selection of blood – 34 the day of the experiment), V – experimental animals which on the 20th day after the formation of the DM introduced the CCP (blood sampling – 34 day of the experiment).

Results of research and discussion. Immunological parameters of blood and functional indicators of phagocytes in diabetes mellitus in animals indicate the presence in their body of certain pathological processes caused by insulin insufficiency. Introduced on the background of diabetes mellitus the CCBM and the CCP reduces the level of glucose in the blood of animals-recipients and contribute to the restoration of blood parameters in the direction of the baseline condition.

Conclusion and prospects for further research. Immunological parameters of blood and functional indicators of phagocytes in diabetes mellitus in animals indicate the presence in their body of certain pathological processes caused by insulin insufficiency. Introduced on the background of diabetes mellitus the CCBM and the CCP reduces the level of glucose in the blood of animals-recipients and contribute to the restoration of іmmunological parameters of blood in the direction of the baseline condition.

Keywords: diabetes mellitus, culture cells of bone marrow, culture cells of the pancreas, glucose, leukocytes, immunoglobulins.

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