Veterynarna biotehnologija – Veterinary biotechnology, 2016, 29, 156-162 [in Ukrainian].

KUDRYAVCHENKO А.P., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., ROMANENKO O.A., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., PUSTOVIT N.A., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., ROMANYSHYNA Y.R., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.

State Scientific Control Institute of Biotechnology and Strains of Microorganisms

CULTIVATION OF TOXOPLASMA GONDII IN CELL CULTURE

Introduction. Spread of Toxoplasma makes this patogen quite dangerous for animals and humans because of the three forms of pathogens (tachyzoites, bradizoites, oocysts).

The goals of the work were to maintain vitality, accumulate Toxoplasma gondii tachyzoites in vitro and create a positive control for polymerase chain reaction.

Materials and methods. The cell lines Vero, CrFK, HeLa for Toxoplasma gondii cultivation were used.

Results of research and discussion. It was established that cell lines usage as producers in a stage of rapid growth, allows to increase the concentration of Toxoplasma gondii tachyzoites in 50 times for 120 hours, which makes it possible to maintain the viability of toxoplasmosis pathogen in the laboratory.

Conclusions and perspectives for further research. The use of Vero, CrFK, HeLa cell lines in a stage of rapid growth, allows increasing the concentration of Toxoplasma gondii tachyzoites in 50 times for 120 hours. The value of the method of cultivation is its ability to maintain the viability of toxoplasmosis pathogen in the laboratory conditions. Significant role in toxoplasmosis spreading is played by cats. They are the definitive host of the pathogen that distribute oocysts with the feces, contaminate the environment and are the subject of a high risk of human infection therefore toxoplasmosis study indicates the need for further disease monitoring in Ukraine.

Keywords: diagnosis, tachyzoites, toxoplasmosis, cell culture, oocysts.

REFERENCES

1. Aksman, A. (1985). Priobretennyy toksoplazmoz s porazheniem tsentral’noy nervnoy sistemy [Acquired toxoplasmosis with central nervous system lesions]. Meditsinskaya parazitologiya – Medical parazitology, 5, 18-21 [in Russian].
2. Akbaev, M.S. (1998). Parazitologija i invazionnye bolezni zhivotnyh [Parasitology and parasitic animal dieses]. Moskow [in Russian].
3. Galat, V.F., Berezovs’kij, A.V., Prus, M.P., & Soroka, N.M. (2003). Parazitologija ta invazijni hvoroby tvarin [Parasitology and invasive dieses of animals]. Kyi’v: Vyshha osvita [in Ukrainian].
4. Beyer, T.V. Svezhova, N.V., Radchenko, A.I.,. & Sidorenko, N.V. (2002). Parasitophorous vacuole: morphofunctional diversity in different coccidian genera (a short insight into the problem). Cell Biology International, 26, 861-871.
5. Dubey, J.P. (2009). History of the Discovery of the Life Cycle of Toxoplasma gondii. International Journal for Parasitolog, 39, 877-882.

Download full text in PDF