Veterynarna biotehnologija – Veterinary biotechnology, 2016, 29, 204-212 [in Ukrainian].

PETRENKO O.S., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., ALEKSEEVA H.B., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., PRISKOKA V.A., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., KYIVSKA G.V., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.

State Scientific and Research Institute of Laboratory Diagnostics and Veterinary and Sanitary Expertise

GOLOVAHA V.I., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.

Bila Tserkva National Agrarian University

COMPARATIVE CHARACTERISTICS OF THE SENSITIVITY OF THE ELISA TEST SYSTEMS OF DIFFERENT MANUFACTURERS FOR DIAGNOSIS OF ENZOOTIC BOVINE LEUKOSIS

Introduction. Using pooled milk samples, as an object of immunological diagnosis of bovine leukemia ELISA requires a reliable assessment of the sensitivity of the ELISA test system as unreliable diagnosis poses a threat to the welfare of epizootic.

The goal of the work was to determine and compare the sensitivity of ELISA test kits for the diagnosis of bovine leukosis of different manufacturers with using serial dilutions of a positive sample of milk as a secondary intralaboratory reference sample.

Result of research and discussion. Low positive reference sample is the critical factor that contributes to guarantee the diagnostic sensitivity of the investigation. Only when it is used it can be ensured that suitable immunological method is used to detect a certain level of specific antibodies. Using a prefabricated milk sample as the object of immunological diagnosis of bovine leukosis, even substantial dilutions a separate sample in a pool of milk, is cost-effective in prosperous farms and ensures high-quality diagnosis of the disease and reducing the cost of monitoring studies (through the use of a secondary standard instead of an international standard serum E05).

Conclusion and prospects for further research. Specialized test system BLV Milch-S (Institut Pourquier SAS) and Leukosis Milk Screening (IDEXX Montpellier SAS) are highly sensitive and can be used as a means of qualitative diagnosis of leukosis, even at high dilutions of a single sample. Prospect for further research is the development of secondary reference sample by comparative research of international standard serum E05, and implementation of regional network of state and regional veterinary laboratories weakly positive standardized samples for continuous monitoring of the analytical performance of immunological research methods (RID and ELISA) on bovine leukosis.

Keywords: leukosis, cattle, ELISA, milk, immunological studies, validation, methodologies, characterization, analysis, sample, quality.

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