Bulletin "Veterinary biotechnology"

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Veterynarna biotehnologija – Veterinary biotechnology, 2016, 29, 233-241 [in Ukrainian].

RYZHENKO G.F., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., GORBATYUK O.I., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., ANDRIYASCHUK V.O., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., ZHOVNIR O.M., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., UKHOVSKA T.M., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., TIUTIUN S.M., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., KAMENCHUK P.P., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.

Institute of Veterinary Medicine of the NAAS

MICROBIOLOGICAL CONTAMINATION OF MILK AND MILK PRODUCTS AT SUBCLINICAL MASTITIS IN COWS AND WAYS OF ITS PREVENTION

Introduction. Pathogens causing subclinical mastitis are predominantly antibiotic-resistant microflora, so the immunization of mastitis in cows remains relevant.

The goal of the work was to investigate the microorganism species spectrum in cows with subclinical mastitis; to study isolates’ sensitivity to antibiotics; propose specific means of cow’s subclinical mastitis prevention.

Materials and methods. Samples of cow’s milk were selected on farms LLC SPA “Perlyna Podillja” in Jurivka, Zhyzhnykivci and livestock farms in different regions of Ukraine. Studying of the vaccine “Pnevmomastysan” samples effect on the cattle organism under production conditions was conducted in 19 livestock farms of Ukraine; vaccine “Multybovisan” in 2 farms of Chernihiv oblast; preparation “STP” in the farms of Rivne oblast. We used bacteriological, biological, immunological methods and conducted economic analysis.

Results of researches and their discussion. Studies have shown that subclinical mastitis in cows and heifers caused by pathogens associations. The main representatives of microbial associations at subclinical mastitis were Streptococcus spp. (73.5% of cases in associations); Clostridia (54.4% of cases in associations) among which the rate of C. perfringens was 33.8% and Clostridium spp. was 20.6%; Staphylococci (47.1% of cases in associations) among which S. aureus was isolated in 26.5% of cases and Staphylococcus spp. – in 20.6%; Klebsiella spp. – 14.7% and Ps. aeruginosa – 4.4% of cases in associations. Results of antibiotic resistance studies of the isolates showed their sensitivity to antibiotics in 17.2–31.0% of cases. Associated inactivated vaccine “Pnevmomastysan” against pneumoenteritis, endometritis and mastitis animals was tested in 19 farms in different regions of Ukraine. Economic efficiency after associated vaccine “Multibovisan” applying was about 800 thousand hrivnia. Applying of preparation “STP” showed a positive effect on immunobiological reactivity of the organism and the formation of strong immunity in vaccinated animals.

Conclusions and prospects for further research. It was revealed that the main representatives of microbial associations at subclinical mastitis were Streptococcus spp. (73.5%); Clostridia (54.4%), where C. perfringens was 33.8% and Clostridium spp. was 20.6%; Staphylococci (47.1%), where S. aureus was isolated in 26.5% of cases and Staphylococcus spp. in 20.6%; Klebsiella spp. – 14.7% and Ps. aeruginosa – 4.4%. Established that isolated pathogens’ cultures were sensitive to antibiotics in 17.2–31.0% of cases. Associated inactivated vaccine “Pnevmomastysan” against pneumoenteritis, endometritis and mastitis animals was tested in 19 farms in different regions of Ukraine. Economic efficiency after associated vaccine “Multibovisan” applying was about 800 thousand hrivnia. Applying of preparation “STP” showed a positive effect on immunobiological reactivity of the organism and the formation of strong specific immunity in vaccinated animals. The results showed the need to develop new means of specific prevention of bacterial infections of animals using metal nanoparticles.

Keywords: mastitis, microbial associations, veterinary immunobiological means.

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