Bulletin "Veterinary biotechnology"

  • Print

Veterynarna biotehnologija – Veterinary biotechnology, 2016, 29, 253-265 [in Ukrainian].

TIUTIUN S.V., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., RYZHENKO G.F., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., GORBATIUK O.I., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., ANDRIIASCHIUK V.A., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., ZHOVNIR O.M., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.

Institute of Veterinary Medicine of the NAAS

VACCINE PREVENTION AND VACCINE THERAPY AT CATTLE FUSOBACTERIOSIS (NECROBACILLOSIS)

Introduction. The paper highlights the issue of vaccine prevention and vaccinotherapy at cattle fusobacteriosis using vaccine “Nekrosan”, and in case of associated infections – “Nekrosan-2”, “Nekrosan-3”, “Nekrosalm”, “Nekrokolisalm”, “Fuzoaktynosan”. Immunomodulatory agents of plant and animal origin are include in the composition of these vaccines using which you can vaccinate weak and sick animals. This is especially important during the scheduled activities on the farm concerning animals fusobacteriosis prevention or elimination.

The goal of the work. Deep introduction for farm workers, specialists of veterinary medicine, scientists with veterinary immunobiological agents against fusobacteriosis that produced using modern technologies and can be used as for prophylactic so therapeutic purposes.

Materials and methods. Produced vaccines “Nekrosan-2”, “Nekrosan-3”, “Nekrosalm”, “Nekrokolisalm”, “Fuzoaktynosan” were tested by all required physical and biological properties under the current technical specifications of Ukraine for each vaccine and meet their characteristics and standards. Vaccine “Nekrosan” was used with preventive and therapeutic purposes in farms: SF “Matusivskyi” in Cherkasy oblast, “Chaikin” branch of AF “Svitanok” in Chernihiv oblast, EF “Askaniyske” in Kherson oblast, EF “Lidiyevske” in Mykolaiv oblast, EF “Elitne” in Kyiv oblast, Tsyurupa’s PAE in Zhytomyr oblast, etc. Results of research and discussion. In Ukraine, the first time vaccine “Nekrosan” was produced in 1998, and testing of five vaccines has been performed in recent years: “Nekrosan-2”, “Nekrosan-3”, “Nekrosalm”, “Nekrokolisalm”, “Fuzoaktynosan”. Animal vaccination provides: - strong long-term protection of animal (up to 80–90%) against fusobacteriosis; - mild course and clinical manifestations of the disease; - accelerates the recovery of sick animals; - eliminating or reducing the time of antibiotic treatment, which is particularly important for dairy cattle; - reduction of the term of treatment of affected limbs in 2–3 times and accelerate the recovery of sick animals; - reduce the cost of animals’ treatment; - prolongation of the use of cows for 1–2 lactation, which contributed to obtaining additional 10–12 thousand. kg of milk per cow; - decrease of the highly productive cows culling rate; - increasing of cows’ reproductive function. Personal experience shows that applying our vaccines against fusobacteriosis and fusobacteriosis complicated by associated infections can be vaccinated as sick animals so weakened ones because they include immunomodulatory agents of plant and animal origin.

Conclusions and prospects for further research. For specific prevention of fusobacteriosis in cattle vaccine “Nekrosan”should be used. Depending on the epizootic state it is allowed to vaccinate cattle against fusobacteriosis and infections associated with this disease by inactivated vaccines: “Nekrosan-2” – against fusobacteriosis (necrobacillosis), necrotic hepatitis, malignant edema, infectious (anaerobic) enterotoxemia and colibacteriosis of animals; “Nekrosan-3” – against foot rot, fusobacteriosis (necrobacillosis), infectious (anaerobic) enterotoxaemia and cocci infections of animal; “Nekrosalm” – against necrobacillosis and salmonellosis of animals; “Nekrokolisalm” – against necrobacillosis and colibacillosis and salmonellosis of animals; “Fuzoaktynosan” – against fusobacteriosis and actinobacillosis. Given that these vaccine includes immunomodulatory means of plant and animal origin, vaccination can be used for weak and sick animals. Further studies will be directed on search for advanced technologies of associated vaccines designing against fusobacteriosis and its associated infections – application of advanced colloidal solutions of metals nanoparticles (CuNP, АgNP, АuNP, FеNP).

Keywords: fusobacteriosis, vaccine prevention, vaccine therapy, mono- and associated vaccine, immunomodulating agents.

REFERENCES

  1. Samolovov, A.A., & Lopatyn S.V. (2001). Nekrobakterioz krupnogo rogatogo skota i puti reshenija problemy [Necrobacillosis of cattle and ways of the issue solutions]. Agrarnaja Rossija – Agricultural Russia, 3, 34-37 [in Russian].
  2. Skorodumov, D.I. (2007). Mikrobiologicheskaja diagnostika nekrobakterioza [Microbiological diagnosis of necrobacillosis]. Veterinarija s/h zhivotnyh – Veterinary of farm animals, 12, 21-25 [in Russian].
  3. Veterynarna medycyna. Metody laboratornoi' diagnostyky fuzobakteriozu (nekrobakteriozu) tvaryn Veterinary medicine [Methods of laboratory diagnostics of fusobacteriosis (necrobacillosis) in animals]. (2006). Standart organizacii' Ukrai'ny 85.2-37-510:2006, Kyiv [in Ukrainian].
  4. Varohin, A., Ostrovskij, M., Saharova, E., & et al. (2009). Sankt-Peterburgskaja shema bor'by s nekrobakteriozom KRS [St. Petersburg scheme of cattle necrobacillosis control]. Veterinarija s/h zhivotnyh – Veterinary of farm animals, 4, 25-27 [in Russian].
  5. Ostrovskij, M.V. (2007). Novyj podhod k lecheniju nekrobakterioza KRS [A new approach to the treatment of cattle necrobacillosis]. Veterinarija s/h zhivotnyh – Veterinary of farm animals, 12, 18-20 [in Russian].
  6. Ryzhenko, V.P., Obrazhej, A.F., Ryzhenko, G.F., Dement'jeva, S.A., Ryzhenko, V.V., Bjelik, S.M., & Galka I.V. e.a. Vakcyna “Nekrosan” asocijovana inaktyvovana koncentrovana proty nekrobakteriozu, nekrotychnogo gepatytu, zlojakisnogo nabrjaku ta infekcijnoi' (anaerobnoi') enterotoksemii' tvaryn [Concentrated associated inactivated vaccine “Nekrosan” against necrobacillosis, necrotic hepatitis, malignant edema and infectious (anaerobic) enterotoxemia of animals]. Patent Ukraine, no 12946 А Ukrai'na MPK 7 A61K 39/116, 39/08, 39/114.
  7. Ryzhenko, V.P., Ryzhenko, G.F., Dement'jeva, S.A., Bjelik, S.M., Kamenchuk, P.P., & Hmel'nyc'ka, N.M. e.a. Vakcyna “Nekrosan-2” asocijovana inaktyvovana koncentrovana proty nekrobakteriozu (fuzobakteriozu), nekrotychnogo gepatytu, zlojakisnogo nabrjaku, infekcijnoi' (anaerobnoi') enterotoksemii' ta kolibakteriozu tvaryn [Concentrated associated inactivated vaccine “Nekrosan 2” against necrobacillosis (fusobacteriosis), necrotic hepatitis, malignant edema and infectious (anaerobic) enterotoxemia and colibacteriosis of animals]. Patent Ukraine, no 18334 А Ukrai'na, МПК 7А61К 39/00.
  8. Ryzhenko, V.P., Obrazhej, A.F., Ryzhenko, G.F., Gorbatjuk, O.I., Andrijashhuk, V.O., Mil'ko, L.S., & Bjelik, S.M. e.a. Vakcyna “Nekrosan-3” asocijovana inaktyvovana koncentrovana proty kopytnoi' gnyli, nekrobakteriozu (fuzobakteriozu), infekcijnoi' (anaerobnoi') enterotoksemii' ta kokovyh infekcij tvaryn [Concentrated associated inactivated vaccine “Nekrosan 3” against hoofed rot, necrobacillosis (fusobacteriosis), infectious (anaerobic) enterotoxemia and coccal infections of animals]. Patent Ukraine, no № 50014 Ukrai'na, МPК (2010) GO1N33/00.
  9. Ryzhenko, V.P., Ryzhenko, G.F., Kucherjavenko, O.O., Dement'jeva, S.A., Andrijashhuk, V.O., Galka, I.V., & Zhovnir, O.M. e.a. Vakcyna “Nekrosal'm” inaktyvovana koncentrovana proty nekrobakteriozu (fuzobakteriozu) ta sal'monel'ozu tvaryn [Concentrated inactivated vaccine “Nekrosalm” against necrobacillosis (fusobacteriosis) and salmonellosis of animals]. Patent Ukraine, no 18335 А Ukrai'na, МPК 7А61К 39/00.
  10. Ryzhenko, V.P., Ryzhenko, G.F., Kucherjavenko, O.O., Dement'jeva, S.A., & Andrijashhuk, V.O. e.a. Vakcyna “Nekrokolisal'm” asocijovana inaktyvovana koncentrovana proty nekrobakteriozu (fuzobakteriozu), kolibakteriozu ta sal'monel'ozu tvaryn [Concentrated associated inactivated vaccine “Nekrokolisalm” against necrobacillosis (fusobacteriosis), colibacillosis and salmonellosis of animals]. Patent Ukraine, no 18332 А Ukrai'na, МPК 7А61К 39/00.
  11. Ryzhenko, V.P., Ryzhenko, G.F., Kucherjavenko, O.O., Andrijashhuk, V.O., Galka, I.V., & Ryzhenko, V.V. e.a. Vakcyna “Fuzoaktynosan” inaktyvovana koncentrovana asocijovana proty nekrobakteriozu (fuzobakteriozu) i aktynobacyl'ozu tvaryn [Concentrated associated inactivated vaccine “ Fuzoaktynosan” against necrobacillosis (fusobacteriosis) and actinobacillosis of animals]. Patent Ukraine, no 25635 А Ukrai'na, МPК 7А61К 39/114, 39/00.
  12. Rizhenko, V.P., Rizhenko, G.F., & Gorbatjuk, O.І. et al. (2009). Biotychni vidnosyny Fusobacterium necrophorum iz asociacijamy anaerobnyh i aerobnyh mikroorganizmiv [Biotic relations of Fusobacterium necrophorum with associations of anaerobic and aerobic microorganisms]. Veterynarna biotehnologija – Veterinary biotechnology, 14, 278–285 [in Ukrainian].
  13. Rizhenko, V.P., Rizhenko, G.F., & Gorbatjuk, O.І. et al. (2009). Stan ta perspektyvy udoskonalennja specyfichnoi' profilaktyky fuzobakteriozu (nekrobakteriozu) [State and prospects of improvement of fusobacteriosis (necrobacillosis) specific prevention]. Veterynarna biotehnologija – Veterinary biotechnology, 14, 253-266 [in Ukrainian].
  14. Integral'na systema zahystu tvaryn vid fuzobakteriozu (nekrobakteriozu) [The integrated system of animals’ protection from fusobacteriosis (necrobacillosis)]. (2011). Manual. Kyiv: CP “KOMPRYNT” [in Ukrainian].
  15. Metody patogenetychnoi' terapii' i profilaktyky fuzobakteriozu (nekrobakteriozu) velykoi' rogatoi' hudoby [The methods of pathogenetic therapy and prophylaxis of fusobacteriosis (necrobacillosis)]. (2012). Manual. Kyiv: CP “KOMPRYNT” [in Ukrainian].

Download full text in PDF