Veterynarna biotehnologija – Veterinary biotechnology, 2016, 29, 266-270 [in Ukrainian].

HOMENKO I.V., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.

Institute of Veterinary Medicine of the NAAS

THE METHOD FOR DIFFERENTIATION OF ANTIBODIES SPECIFIC FOR YERSINIA ENTEROCOLITICA (SEROVAR 0:9) AND BRUCELLA ABORTUS

Introduction. Results of evaluation of dot-blot immunoassay for differentiation of antibodies, specific to B. аbortus or Yersinia enterocolitica (serovar 0:9). The protocol of immunoassay includes simultaneous sorption of B. аbortus and Yersinia enterocolitica antigens on polysterene comb. Recombinant protein G conjugated with colloid gold was used as conjugate.

The goal of the work. To develop the dot-blot immunoassay to distinguish antibodies specific for Yersinia enterocolitica (serovar 0:9) and Brucella аbortus.

Materials and methods. The main principle of suggested method is immunologic reaction between antibody and antigen. Immune complexes are detected with recombinant protein G (Streptococcus spp.) conjugated with colloid gold. The protocol of reaction is based on dot-blot immunoassay. For sensibilisation of polysterene combs lipopolysaccharides of B. аbortus аnd Yersinia enterocolitica (serovar 0: 3) were used. In rhe investigation the next control materials were used: sera of animals, which are positive or negative for brucellosis obtained from MEB reference-center, sera of rabbits immunized with different serovars of Yersinia enterocolitica, sera of nonimmunized rabbits.

Results of the study and discussion. During investigation of sera of animals, which are positive for brucellosis specific reaction was detected with B. аbortus antigen and no reaction was detected with Yersinia enterocolitica antigen. Hyperimmune rabbit serum to Yersinia enterocolitica (serovar 0:9) reacted with both Yersinia enterocolitica and B. аbortus antigens. Sera of rabbits immunized with another serovars of Yersinia enterocolitica specific reaction was detected with antigen of Yersinia enterocolitica (serovar 0:3).

Conclusions and prospects for further research. Dot-blot immunoassay based on simultaneous sorption of Yersinia enterocolitica (serovar 0:9) and Brucella аbortus antigens was developed to differentiate brucellosis and yersiniosis.

Keywords: brucellosis, yersiniosis, colloidal gold, protein G, dot-blot immunoassay.

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