Bulletin "Veterinary biotechnology"

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Veterynarna biotehnologija – Veterinary biotechnology, 2018, 33, 55-65 [in Ukrainian]. https://doi.org/10.31073/vet_biotech33-07

KOVPAK O., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., KOVPAK V., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., MAZURKEVICH A., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.

National University of Life and Environmental Sciences of Ukraine

HUDZ N., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.

Institute of Veterinary Medicine of the NAAS

EFFECT OF FIBROBLASTS GROWTH FACTOR (FGF-2) AND INSULIN-LIKE GROWTH FACTOR (IGF-1) ON THE PROLIFERATIVE ACTIVITY OF STEM CELLS OF CAT

Introduction. Сell technologies implementation in clinical practice requires a large amount of cellular material. This is stimulates the improvement of cultivation conditions, which will allow to obtain more cellular material over a shorter period of time. From literature data, it is known that fibroblast growth factor (FGF-2) and insulin-like growth factor (IGF-1) can positively affect the mitotic activity of stem cells. Therefore, the goal of the work was to investigate the effect of fibroblast growth factor (FGF-2) and insulin-like growth factor (IGF-1) in various concentrations on proliferative activity and genetic stability of stem cell cultures derived from bone marrow, adipose tissue and cat's myocardium.

Materials and methods. In research we used third passage of stem cells of fat tissue, bone marrow and myocardium. Passage was carried out in the 1:5 dilution. Cells were cultured in a standard medium: 80% DMEM; 20% – FBS; 10 μl/cm3 – antibiotic-antimycotic with the addition of: 1. Insulin-like growth factor (IGF-1) at concentration of 10, 20 and 50 ng/ml; 2. Fibroblast growth factor (FGF-2) at concentration of 10, 20 and 50 ng/ml; 3. Control (cultivation in a standard culture medium). Results of research and discussion. It was found that the FGF-2 has a positive effect on the proliferative activity of stem cells in cell cultures of adipose tissue, cardiac muscle and bone marrow of cat at low concentrations (10 ng/ml). IGF-1 has a positive effect on the proliferative activity of stem cells in cell cultures of bone marrow and cardiac muscle of cat at high concentrations (50 ng/ml), while the effect on the cell culture of adipose tissue stem cells was the opposite. According to the cytogenetic analysis it was found that adding FGF-2/IGF-1 to the culture media does not lead to a significant increase in the number of genetic errors in all the studied cell cultures.

Conclusion and prospects for further research. It was established that IGF-1 and FGF-2 positively influence the proliferative activity of all studied cultures. According to the cytogenetic analysis, it was determined that the addition of growth factors to the culture medium does not lead to a significant increase in the number of genetic errors (in comparison with the control) in all the studied cultures. The results obtained in the research will be used for the introduction of cell technologies into veterinary practice.

Keywords: fibroblast growth factor (FGF-2), insulin-like growth factor (IGF-1), stem cells, bone marrow cell culture, adipose tissue culture, myocardial cell culture, cats, cytogenetic analysis.

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