Veterynarna biotehnologija – Veterinary biotechnology, 2018, 33, 88-93 [in Ukrainian]. https://doi.org/10.31073/vet_biotech33-11
MUZYKA V.P., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., LEVYTSKY T.R., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., NAZAR B.I., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.
State Scientific and Research Control Institute of Veterinary Medicinal Products and Feed Additives
MANDYHRA U.M., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.
Experimental Station of Epizootology of the Institute of Veterinary Medicine of NAAS
USE OF MOLECULAR-BIOLOGICAL METHODS OF RESEARCHES FOR NON-ADMISSION OF ORIGIN PRION INFECTIONS IN UKRAINE
Introduction. Use of the gene technology to analyze the quality of food products for humans and animal feed is required. For this purpose sensitive, fast and accurate method could be used to detect and prevent falsifications, in particular, falsified food products, animal feeds and food additives, the actual composition of which does not correspond to the declared and to the labeling information. Application of the screening and confirmatory methods for detecting falsified food products, animal feeds and feed materials will provide efficient and prompt detection falsification cases, to prevent circulation of low-quality food products and feeds, decreasing the productivity and poisoning of animals, and consequently to obtain safe and high-quality livestock products.
The goal of the work was to the prevent cases prion infections, namely identification of animal proteins using histological and PCR methods that allow differentiate the constituents of food, because meat products, and the raw material for feed of animal origin at the stage of technological processing mainly, do not retain their morphological features.
Materials and methods. The researches were carried out using microscopic analysis with the help of confirmation of positive results by polymerase chain reaction method.
Results of research and discussion. Within the framework of state monitoring during 2012–2017, the State Scientific- Research Control Institute of Veterinary Medicinal Products and Feed Additives conducted the quality control of feed for non-productive animals, premixes, feed additives, vitamins, including the determination of the presence of proteins of ruminants. The presence of proteins of ruminants was found in 64 out of 402 samples of feed supplements for non-productive animals, which were submitted for the registration in Ukraine. Besides this, these proteins was found in 12 samples of other products (minced meat of various origin, soup, veterinary preparations) when trying to import to Ukraine. To prevent the spread of prion infections in Ukraine, the registration of these products with the further shipment to Ukraine, was denied.
Conclusions and prospects for further research. The application of an integrated approach to the recognizing of animal components in feed and food is an effective mechanism for preventing prion diseases. The use of the PCR method for confirmation of the presence and identification of species of animal tissues allows to prevent the falsification of products, to differentiate the species belonging of proteins in the composition of feed for animals and meat products, that presents an important economic and epidemiology value, and in future will warn the emergence of prion infections which are dangerous as for people so for animals.
Keywords: molecular research methods, PCR method, species identification of the protein of animal and vegetable sources.
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