Veterynarna biotehnologija – Veterinary biotechnology, 2018, 33, 93-98 [in Ukrainian]. https://doi.org/10.31073/vet_biotech33-12
PISHCHANSKYI O., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., SAPACHOVA M., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., SUSHKO M., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., USACHENKO N., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., MEZHENSKYI A., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., KYIVSKA G., e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.
Державний науково-дослідний інститут з лабораторної діагностики та ветеринарно-санітарної експертизи
MOLECULAR GENETICS MONITORING OF INFLUENZA IN WILD AND DOMESTIC BIRDS IN UKRAINE IN 2016−2017
Introduction. Continuous monitoring of the Avian influenza (AI) is carried out with the aim of studying of epizootic situation, the timely detection of infected birds and the elimination of outbreaks of the disease. Molecular genetics monitoring of AI in Ukraine, that is a subject of research, is an important part of the epizootic surveillance of the disease. Main areas for sampling are places of seasonal accumulation of wild and migratory birds, which are carriers of the virus.
The goal of the work was to study the epizootic state concerning of AI in Ukraine in 2016−2017.
Materials and methods. Tests of the pathological / biological material from the birds and typing of AI virus were carried out by PCR-RT. At first, the detection of AI RNA virus (detection of the M-gene) was performed. If the sample was positive for AI, the subtyping on haemagglutinins H5, H7 and neuraminidase N8 was carried out. Samples containing AI virus type A subtype H5N8 were submitted to OIE reference laboratory for AI pathogenicity determination and full genome sequencing.
Results of research and discussion. In 2016−2017 5789 samples of pathological / biologicals materials were tested, 2434 samples from wild birds and 3355 samples from domestic ones. AI virus type A subtype H5N8 was detected in 51 samples: 17 samples from wild birds, and 34 samples from domestic birds. Comparison of RNA nucleotide sequences of Ukrainian isolates of influenza A / H5N8 virus from domestic and wild birds with viruses that were isolated in countries of Europe and Asia during 2016–2017. indicates a high level of homology. Sequences of amino acids in the cleavage site of hemagglutinin confirm that the Ukrainian isolates of the influenza A / H5N8 virus are highly pathogenic.
Conclusions and prospects for further research: 1. In 2016−2017 outbreaks of AI were registered in Ukraine. The disease was caused by highly pathogenic AI virus type A subtype H5N8. 2. It was found that isolated viruses belong to type A subtype H5N8. 3. Territorial analysis shows that the virus spreads to the south-western and southern regions of Ukraine. 4. The results of the studies confirm the need for continuous monitoring of AI, especially among wild migratory birds and sinanthropic birds, in order to detect infection in a timely manner, to prevent the spread of AI in poultry and to control the epizootic situation.
Keywords: Avian influenza, diagnostics, epizootic situation, monitoring.
REFERENCES
